Learn More
BACKGROUND Diabetes mellitus (DM) is increasing in men of reproductive age. Despite this, the prevalence of diabetes in men attending fertility clinics is largely unknown. Furthermore, studies examining the effects of DM on sperm fertility potential have been limited to conventional semen analysis. METHODS Conventional semen analysis (semen volume, sperm(More)
The aim of this study was to determine the effects of supplementation with ascorbate and alpha-tocopherol, both singly and in combination, during sperm preparation on subsequent sperm DNA integrity, induced DNA damage and reactive oxygen species (ROS) generation. Semen samples with normozoospermic and asthenozoospermic profiles (n = 15 for each control and(More)
OBJECTIVE To determine the presence and localization of leukocyte subsets in the human follicular wall and in the corpus luteum (CL) throughout the menstrual cycle. DESIGN Tissue was obtained by biopsy at laparoscopy. Frozen sections were labeled with a panel of monoclonal antibodies against leukocyte subtype markers and detected by immunohistochemical(More)
Sperm DNA integrity is essential for accurate transmission of genetic material to offspring. Fragmentation of genomic DNA is an initial hallmark of apoptosis (programmed cell death). The aim of this study was to determine sperm nuclear DNA integrity and mitochondrial function, to quantify possible apoptosis and to investigate any relationship between these(More)
OBJECTIVE To investigate effects of cryopreservation on sperm motility and DNA integrity. DESIGN Pre-cryopreservation and post-cryopreservation analysis of motility and DNA integrity of semen and prepared sperm samples. SETTING A hospital andrology laboratory. PATIENT(S) Forty men attending the Regional Fertility Centre, Belfast, Northern Ireland. (More)
OBJECTIVE To determine the effects of supplementation of preparation media with ascorbate and alpha-tocopherol on subsequent sperm motility and reactive oxygen species production. DESIGN Prospective study to analyze postpreparation human sperm motility parameters and reactive oxygen species production following antioxidant supplementation. SETTING(More)
BACKGROUND The loss of three-dimensional visual depth cues has been thought to be the main hindrance to the acquisition of endoscopic skills. However, the "fulcrum effect" of the abdominal wall on the manipulation of instruments has not to our knowledge been previously investigated. METHODS Subjects with no experience in endoscopy were required to make(More)
BACKGROUND The "fulcrum effect" of the body wall on surgical instrument manipulation is a major hurdle for novice endoscopic surgeons. Virtual reality training has not previously been evaluated as a means to overcome this problem. MATERIALS AND METHODS 16 participants with no experience of endoscopy were required to make multiple defined incisions under(More)
BACKGROUND The effects of cryoinjury were determined simultaneously on the mitochondrial function, motility, morphology and viability of ejaculated human sperm. METHOD Rhodamine 123 (R123) uptake (% of sperm) and stain intensity were used to determine sperm mitochondrial activity before and after cryopreservation from the semen of 50 men attending for(More)
Light microscopic studies comparing sperm parameters show little association between diabetes and male fertility. However, with the introduction of new analytical techniques, evidence is now emerging of previously undetectable effects of diabetes on sperm function. Specifically, a recent study has found a significantly higher sperm nuclear DNA fragmentation(More)