Neelawan Pongsilp

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Fourteen root-nodule bacteria isolated from the medicinal legume Indigofera tinctoria were characterized for their phenotypic features including growth curves, utilization of carbon and nitrogen sources, antibiotic resistance, vitamin requirement and growth under different conditions. The partial sequences of the 16S rDNA of these strains were obtained and(More)
A total of 215 root-nodule bacteria were isolated from 3 medicinal legumes including Indigofera tinctoria, Pueraria mirifica and Derris elliptica Benth. naturally grown in 16 provinces of Thailand. The isolates were evaluated for DNA polymorphism using randomly amplified polymorphic DNA (RAPD) analysis. Isolates generated 92 identical RAPD profiles,(More)
Denaturing Gradient Gel Electrophoresis (DGGE) was used to study the impact of rhizobial inoculants on the rhizosphere bacterial communities of three medicinal legumes: Indigofera tinctoria, Pueraria mirifica and Derris elliptica Benth. Rhizosphere soils were collected from these legumes grown naturally in 11 provinces of Thailand. The host-specific(More)
Bacterial strains from inoculated soybean field soil in Thailand were directly isolated using Bradyrhizobium japonicum selective medium (BJSM), on the basis of Zn(2+) and Co(2+) resistance of B. japonicum and B. elkanii. The isolates were classified into symbiotic and non-symbiotic groups by inoculation assays and Southern hybridization of nod and nif(More)
One hundred and forty-two Bradyrhizobium strains were screened for their ability to produce N-acyl homoserine lactone-like molecules (AHLs) by using an Agrobacterium tumefaciens biosensor strain containing a traI-lacZ fusion. Approximately 22% (31 of 142) of the tested strains produced AHLs that induced moderate to elevated β-galactosidase activity levels(More)
The rationale of this study was to determine effects of root exudates on soil bacterial community within a particular period as well as on genetic diversity and phenotypic features of culturable bacteria. Total DNA was extracted directly from 3 soil samples including bulk soil, Brassica oleracea rhizosphere and Phaseolus vulgaris rhizosphere. Denaturing(More)
The genotypic diversity of rhizospheric bacteria of 3 legumes including Vigna radiata, Arachis hypogaea and Acacia mangium was compared by using cultivation-dependent and cultivation-independent methods. For cultivation-dependent method, Random amplified polymorphic DNA (RAPD) profiles revealed that the bacterial genetic diversity of V. radiata and A.(More)
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