Nathan M. Belliveau

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Limit size systems are defined as the smallest achievable aggregates compatible with the packing of the molecular constituents in a defined and energetically stable structure. Here we report the use of rapid microfluidic mixing for the controlled synthesis of two types of limit size lipid nanoparticle (LNP) systems, having either polar or nonpolar cores.(More)
Lipid nanoparticles (LNP) containing ionizable cationic lipids are the leading systems for enabling therapeutic applications of siRNA; however, the structure of these systems has not been defined. Here we examine the structure of LNP siRNA systems containing DLinKC2-DMA(an ionizable cationic lipid), phospholipid, cholesterol and a polyethylene glycol (PEG)(More)
Lipid nanoparticles (LNP) are the leading systems for in vivo delivery of small interfering RNA (siRNA) for therapeutic applications. Formulation of LNP siRNA systems requires rapid mixing of solutions containing cationic lipid with solutions containing siRNA. Current formulation procedures employ macroscopic mixing processes to produce systems 70-nm(More)
The androgen receptor (AR) plays a critical role in the progression of prostate cancer. Silencing this protein using short-hairpin RNA (shRNA) has been correlated with tumor growth inhibition and decreases in serum prostate specific antigen (PSA). In our study, we have investigated the ability of lipid nanoparticle (LNP) formulations of small-interfering(More)
A mistake has been found in the article on page 18444. The second paragraph should read: “Here we explored whether limit size particles compatible with such structures could be generated using PEG-lipid as the surface lipid. In this regard a vesicle containing an internal aqueous core of 5 nm diameter has an outside-to-inside surface area ratio of 6.8(More)
Allosteric regulation is found across all domains of life, yet we still lack simple, predictive theories that directly link the experimentally tunable parameters of a system to its input-output response. To that end, we present a general theory of allosteric transcriptional regulation using the Monod-Wyman-Changeux model. We rigorously test this model using(More)
14 Allosteric regulation is found across all domains of life, yet we still lack simple, predictive theories that 15 directly link the experimentally tunable parameters of a system to its input-output response. To that end, 16 we present a general theory of allosteric transcriptional regulation using the Monod-Wyman-Changeux 17 model. We rigorously test this(More)
467 Manuel Razo-Mejia1,†, Stephanie L. Barnes1,†, Nathan M. Belliveau1,†, Griffin Chure1,†, 468 Tal Einav2,†, Rob Phillips1,3,∗ 469 Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, United 470 States; Department of Physics, California Institute of Technology, Pasadena, United States; 471 Department of Applied(More)
A simple, efficient, and scalable manufacturing technique is required for developing siRNA-lipid nanoparticles (siRNA-LNP) for therapeutic applications. In this chapter we describe a novel microfluidic-based manufacturing process for the rapid manufacture of siRNA-LNP, together with protocols for characterizing the size, polydispersity, RNA encapsulation(More)
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