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Acid-base properties of wheat, lupin, pea root cell walls were investigated. The roots of etiolated and green plants of different age were analysed by the potentiometric method. The ion exchange capacity of root cell walls (Si) was estimated at various pH values (pHi 2 to pHi 12) and constant ion strength of the solution (10 mM). To analyse polysigmoid(More)
Acid-base properties and the swelling capacity of wheat, lupin and pea root cell walls were investigated. Roots of seedlings and green plants of different age were analysed by the potentiometric method. The ion exchange capacity (S i) and the swelling coefficient (K cw) of root cell walls were estimated at various pH values (from 2 to 12) and at different(More)
Ion-exchange properties of the polymeric matrix of cell walls isolated from roots of 55-day-old Spinacia oleracea L. (Matador cv.) plants grown in nutrient solution in the presence of 0.5, 150, and 250 mM NaCl and from roots of Suaeda altissima L. Pall plants of the same age grown in the presence of 0.5 and 250 mM NaCl were studied. The ion-exchange(More)
Nonaqueous titration was used for detection of free amino groups in the polymeric matrix of plant cell walls. The content of amino groups varied in the range 0.54–0.91 and total nitrogen in the range 1.0–4.2 mmol per gram dry mass of cell walls depending on the plant species. However, these data on the high content of free amino groups do not correlate with(More)
The swelling capacity of cell walls isolated from different parts of lupine root was investigated. The water content in fragments of intact roots (Q) and swelling coefficient of standardized samples of cell walls (Kcw) were determined, and the dependences of Q and Kcw on the distance from the root tip (L) were plotted. It was shown that the change in Q(More)
Acid–base properties of cell walls isolated from various root tissues of 7-day-old lupine seedlings and 14-day-old lupine plants grown in various media were studied. The ion-exchange capacity of root cell walls was estimated at various pH values (from 2 to 12) and constant ionic strength (10 mM). The parameters determining the qualitative and quantitative(More)
Uptake of a cationic dye (methylene blue) by isolated root cell walls, roots of whole transpiring seedlings, and excised roots was investigated using 7-day-old seedlings of cucumber, maize, and wheat. The number of ionogenic groups per 1 g dry and wet weight of the root cell walls, their swelling capacity (K cw), time-dependence of methylene blue (M cw) ion(More)
Are the carboxyl groups of pectin polymers the only metal-binding sites in plant cell walls? To answer this question we investigated Cu2+ and Ni2+ -exchange capacities of isolated root cell walls of seven plant species. As the ionization degree of carboxyl groups and consequently their ability to bind metal ions is dependent upon the solution pH it is(More)
The composition of ionogenic groups and ion-exchange capacity were studied in the polymeric matrix of cell walls isolated from the pollen grain and tissues of vegetative organs (leaves and stems) of Lilium longiflorum Thunb. The ion-exchange capacity was evaluated at different pH values and ionic strength of 100 mM. In the two-layered pollen wall and the(More)
Ion exchange properties of root cell walls of 50- to 60-day-old plants of Suaeda altissima L., grown in nutrient medium in the presence of different NaCl concentration (0.3, 250, 750 mM) were investigated. For all growth conditions, the ion exchange capacity of the cell walls was estimated at various pH values and at different salt concentrations. The total(More)