Natalja M Raevskaya

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Using in vitro and in silico approaches, we demonstrate that the Hmob3 brain-specific sequence is a part of the extended human MAP1B gene 3'UTR encoded by the portion of the exon 7. Previously we had isolated the 1.4 kb Hmob3 clone (Acc. No Y09836) from the human medulla oblongata cDNA library. In the present paper, we report the RT-PCR detection of(More)
A pNS-cat72 vector was constructed based on the replicative vector pNS2 and reporter gene cat of Tn9 transposone, which encodes chloramphenicol acetyltransferase, in order to clone various gene promoters and to assess their chloramphenicol resistance in Corynebacterium glutamicum strains. The strength of promoters of various genes in the Corynebacterium(More)
Brain-specific human genes were studied over the recent years in the Department of Molecular Bases of Human Genetics, Institute of Molecular Genetics. Clones Hfb1, Hmob3, and Hmob33 were selected from human brain cDNA libraries by differential screening. The clones were sequenced, mapped, and tested for expression in various human tissues. In vitro and in(More)
A genomic clone hybridizing with brain-specific sequence Hfb1 was isolated from a chromosome 5 cosmid library. Hfb1 proved to correspond to a new gene exon which codes for a large 3"-untranslated region of the mRNA for synaptic protein complexin 2. Together with the 985-nt Hfb1 cDNA (EMBL Y15167) isolated previously from a cDNA library of the frontal(More)
We report here on the in vitro and in silico characterization of the organization of the human complexin 2 (CPLX2) gene. This encodes for a protein of 134 amino acid residues, contains five exons, is localized on human chromosome 5q35.3, and spans more than 87 kb. We performed in silico analysis of the CPLX2 5' untranslated region (UTR) and propose an(More)
The expression of genes of a number of neurotrophins, their receptors, and also EGR1—one of the participants in the neurotrophin signal pathways—was investigated in normal human lung tissues, as well as in tumor samples from squamous cell carcinoma and adenocarcinoma. Differential expression of the expression of neurotrophin genes (NGF, BDNF, and NT3) was(More)
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