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Transcription activation by RNA polymerase II is a complicated process driven by combined, precisely coordinated action of a wide array of coactivator complexes, which carry out chromatin-directed activities and nucleate the assembly of the preinitiation complex on the promoter. Using various techniques, we have shown the existence of a stable coactivator(More)
The PBAF subtype of the mammalian chromatin remodeling SWI/SNF complex has wide and diverse functions in transcription regulation and development, being both transcription activator and repressor. However, a mechanism accounting for such functional diversity remains unclear. Human PHF10/BAF45a subunit of the PBAF complex plays an important role in brain(More)
A multisubunit complex that contains the novel evolutionarily conserved transcription factor SAYP was isolated, and its protein composition was determined. SAYP was found to unite two complexes with different functions in transcription activation, namely, the chromatin-remodeling complex PBAP (SWI/SNF) and the general transcription factor TFIID, which is(More)
Using the S2 cell system of Drosophila melanogaster, it was shown that the key protein domain mediating the interaction of TFIID and Brahma transcriptional complexes within the BTFly supercomplex is the evolutionary conserved SAY domain of SAYP. TFIID and Brahma coactivators participated in the reporter gene activation induced by the SAY domain in cellular(More)
SAYP is a dual-function transcription coactivator of RNA polymerase II. It is a metazoan-specific factor with regulated expression that is apparently involved in signaling pathways controlling normal development. In Drosophila, SAYP is maternally loaded into the embryo, participates in cell cycle synchronization in early syncytial embryos, and is(More)
Chromatin remodeling complex PBAF(SWI/SNF) alters the structure of chromatin and controls gene expression. PHF10 is a specific subunit of PBAF complex and is expressed as four isoforms in mammalian cells. We demonstrated that all isoforms are expressed in various human cell types of different histological origins. All four isoforms are extensively(More)
SAYP is a dual-function transcriptional coactivator of RNA polymerase II. It is a metazoan-specific factor involved in different signaling pathways that control normal development. In Drosophila, SAYP is present in the organism from the early stages of development and participates in cell cycle synchronization at the blastoderm stage. SAYP is abundant in(More)
The production of major human heat shock protein Hsp70 (HSPA1A) in a eukaryotic expression system is needed for testing and possible medical applications. In this study, transgenic mice were produced containing wild-type human Hsp70 allele in the vector providing expression in the milk. The results indicated that human Hsp70 was readily expressed in the(More)