Nataliya Barinova

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During transcription initiation by bacterial RNA polymerase, the sigma subunit recognizes the -35 and -10 promoter elements; free sigma, however, does not bind DNA. We selected ssDNA aptamers that strongly and specifically bound free sigma(A) from Thermus aquaticus. A consensus sequence, GTA(C/T)AATGGGA, was required for aptamer binding to sigma(A), with(More)
Bacterial RNA polymerase holoenzyme relies on its sigma subunit for promoter recognition and opening. In the holoenzyme, regions 2 and 4 of the sigma subunit are positioned at an optimal distance to allow specific recognition of the -10 and -35 promoter elements, respectively. In free sigma, the promoter binding regions are positioned closer to each other(More)
We recently described a novel basal bacterial promoter element that is located downstream of the -10 consensus promoter element and is recognized by region 1.2 of the sigma subunit of RNA polymerase (RNAP). In the case of Thermus aquaticus RNAP, this element has a consensus sequence GGGA and allows transcription initiation in the absence of the -35 element.(More)
Highly conserved amino acid residues in region 2 of the RNA polymerase sigma subunit are known to participate in promoter recognition and opening. We demonstrated that nonconserved residues in this region collectively determine lineage-specific differences in the temperature of promoter opening.
Ixodes, persulcatus, I. ricinus, D. reticulatus, D. marginatus, H. concinna were recorded in different regional environmental regions. The northern districts of the region are endemic for tick-borne encephalitis (TBE) and Ixodes tick-borne borreliosis (ITBB). The infection of ticks with TBE virus is 0.25%, that with Borrelia is 10.3% of the total number of(More)
Molecular Cell Immediate Early Publication Copyright ©2006 by Cell Press Published Online June 22, 2006 DOI: 10.1016/S1097276506004163 An Additional Basal Promoter Element Recognized by Free RNA Polymerase σ Subunit Determines Promoter Recognition by RNA Polymerase Holoenzyme Andrey Feklistov, Nataliya Barinova, Anastasiya Sevostyanova, Ewa Heyduk, Irina(More)
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