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Efficient site-specific labeling of proteins via cysteines.
Methods for chemical modifications of proteins have been crucial for the advancement of proteomics. In particular, site-specific covalent labeling of proteins with fluorophores and other moieties hasExpand
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Three-color alternating-laser excitation of single molecules: monitoring multiple interactions and distances.
We introduce three-color alternating-laser excitation (3c-ALEX), a fluorescence resonance energy transfer (FRET) method that measures up to three intramolecular distances and complex interactionExpand
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Lighting up individual DNA binding proteins with quantum dots.
The ability to determine the precise loci and occupancy of DNA-binding proteins is instrumental to our understanding of cellular processes like gene expression and regulation. We propose aExpand
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In vivo assembly and single-molecule characterization of the transcription machinery from Shewanella oneidensis MR-1.
Harnessing the new bioremediation and biotechnology applications offered by the dissimilatory metal-reducing bacteria, Shewanella oneidensis MR-1, requires a clear understanding of its transcriptionExpand
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