Natalia Sandetskaya

Learn More
Targeted enrichment of DNA is often necessary for its detection and characterization in complex samples. We describe the development and application of the novel molecular tool for the specific enrichment of prokaryotic DNA. A fused protein comprising the DNA-binding subunit of the bacterial topoisomerase II, gyrase, was expressed, purified, and immobilized(More)
The molecular detection of microorganisms in liquid samples generally requires their enrichment or isolation. The aim of our study was to evaluate the capture and pre-concentration of bacteria by immobilized particular cationic antimicrobial peptides, called synthetic anti-lipopolysaccharide peptides (SALP). For the proof-of-concept and screening of(More)
A combination of Micro-Electro-Mechanical Systems and nanoscale structures allows for the creation of novel miniaturized devices, which broaden the boundaries of the diagnostic approaches. Some materials possess unique properties at the nanolevel, which are different from those in bulk materials. In the last few years these properties became a focus of(More)
A new automated method for the quantitative determination of ascorbic acid in the substance, injection forms, and tablets has been developed and validated. The proposed method is based on two versions of capillary electrophoresis: (i) capillary zone electrophoresis and (ii) micellar electrokinetic chromatography. The method shows high accuracy,(More)
AIM Processing of the samples in molecular diagnostics is complex and labor-intensive. An integrated and automated platform for sample preparation and nucleic acid-based detection can significantly relieve this burden for the users. RESULTS We present a prototype of a versatile and integrated platform for the detection of pathogens in various liquid(More)
A combination of Micro-Electro-Mechanical Systems and nanoscale structures allows for the creation of novel miniaturized devices, which broaden the boundaries of the diagnostic approaches. Some materials possess unique properties at the nanolevel, which are different from those in bulk materials. In the last years these properties became a focus of interest(More)
Targeted enrichment of DNA is often necessary for its detection in complex samples. We describe the development of a novel molecular tool for the specific enrichment of prokaryotic DNA. A fused protein comprising the DNA-binding subunit of the bacterial topoisomerase II, gyrase, was expressed and immobilized on magnetic particles. The specific affinity of(More)
  • 1