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Stable Protein Sialylation in Physcomitrella
TLDR
Functional complex-type N-glycan sialylation was confirmed via mass spectrometric analysis of a stably co-expressed recombinant human protein and it was proven that Physcomitrella meets wide-ranging biopharmaceutical requirements such as GMP compliance, product safety, scalability and outstanding possibilities for precise genome engineering.
Use of DNA barcoding to distinguish the malaria vector Anopheles neivai in Colombia.
TLDR
A reference 535 bp barcode sequence from a fragment of the mitochondrial gene cytochrome oxidase I (COI) was used as a tool for distinguishing this species from others in the subgenus Kerteszia and indicates genetic variability within the species.
Protonema suspension cultures of the medicinal moss Polytrichum juniperinum
TLDR
This high cell density culture of P. juniperinum is suitable for further studies aimed at exploring and establishing a production platform for high-value secondary metabolites, which has scarcely been studied in this type of biological system.
A synthetic protein as efficient multitarget regulator against complement over-activation
TLDR
The fusion protein MFHR13 comprises SCRs FHR11-2:FH1-4:FH13:FH19-20 and protects sheep erythrocytes from complement attack exhibiting 26 and 4-fold the regulatory activity of eculizumab and human FH, respectively.
Stable Protein Sialylation in Physcomitrella
TLDR
The successful achievement of protein N-glycan sialylation in stably transformed P. patens is presented and functional complex-type N- Glycosylation was confirmed via mass spectrometric analysis of a stably co-expressed recombinant human protein.
Protonema Suspension Cultures of Polytrichum Juniperinum as a Potential Production Platform for Bioactive Compounds
TLDR
Ethyl acetate extract from field-collected moss and dichloromethane extract from protonema suspension cultures showed the greatest cell damage, and exhibited a dose-dependent cytotoxic effect against CHO-K1 cells.
Process engineering of biopharmaceutical production in moss bioreactors via model-based description and evaluation of phytohormone impact
The moss Physcomitrella is an interesting production host for recombinant biopharmaceuticals. Here we produced MFHR1, a synthetic complement regulator which has been proposed for the treatment of
The synthetic protein MFHR13 as efficient multitarget regulator against complement over-activation
TLDR
This research presents a novel and scalable approaches that allow for real-time measurement of the immune response to antibiotics in animals and their application in the context of wound healing.
A synthetic protein as efficient multitarget regulator against complement over-activation
TLDR
A synthetic protein, MFHR13, designed as a multitarget complement regulator that protects sheep erythrocytes from complement attack exhibiting 26 and 4-fold the regulatory activity of eculizumab and human FH, respectively is considered a promising candidate as therapeutic for complement-associated diseases.
Process Engineering of Biopharmaceutical Production in Moss Bioreactors via Model-Based Description and Evaluation of Phytohormone Impact
The moss Physcomitrella is an interesting production host for recombinant biopharmaceuticals. Here we produced MFHR1, a synthetic complement regulator which has been proposed for the treatment of