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The feasibility of using fluorescein-conjugated lectins to visualize and differentiate three fungi commonly involved in ophthalmic mycoses was evaluated. Using a panel of fluorescein-conjugated lectins, Candida albicans, Aspergillus fumigatus, and Fusarium solani were rapidly and reproducibly visualized in in vitro culture isolates, as well as in tissue(More)
PURPOSE To evaluate chemokine expression at various retinal sites after ischemia-reperfusion injury, using reverse transcription-polymerase chain reaction (RT-PCR) analysis of selected tissue obtained by laser capture microdissection. METHODS Retinal ischemia was produced in Lewis rats by increasing intraocular pressure for 75 minutes. At 3, 6, 12, and 24(More)
Specific immunohistochemical localization of superoxide dismutase in ocular tissue of Lewis rats revealed the presence of this enzyme predominantly in the corneal epithelium, endothelium, apical regions of the posterior epithelium of the iris, nonpigmented inner ciliary epithelium, lens epithelium, inner segments of photoreceptor cell layer of the retina,(More)
The widespread use of the Internet and electronic publishing has contributed to the evolution of open-access journals, including those in the field of ophthalmic research. Such peer-reviewed electronic publications are highly desirable since they encourage the rapid publication of basic and clinical research, unlike the traditional paper journals. Timely(More)
PURPOSE Results of a previous study demonstrated the presence of superoxide and nitric oxide in experimental autoimmune uveitis (EAU). These two chemical entities have recently been shown to combine rapidly to form one of the most potent known biologic oxidants, peroxynitrite. As an extension of the previous study, the current study was proposed to(More)
PURPOSE Retinal microglial cells of newborn Lewis rats were isolated and cultured, and the effect of macrophage colony-stimulating factor (M-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), and interferon-gamma (IFN-gamma) on microglial expression of the accessory molecules required for antigen presentation were studied. METHODS Retinal(More)
PURPOSE To characterize optic nerve and retinal changes in a patient with end-stage retinitis pigmentosa (RP) with an implanted active epiretinal array. METHODS A 74-year-old man with end-stage X-linked RP underwent implantation of an epiretinal array over the macula in the right eye and subsequent stimulation until his death at 5 years and 3 months after(More)
PURPOSE Neuroglobin (Ngb) is a vertebrate globin that is predominantly expressed in the retina and brain. To explore the role of Ngb in retinal neuroprotection during ischemia reperfusion (IR), the authors examined the effect of Ngb overexpression in the retina in vivo by using Ngb-transgenic (Ngb-Tg) mice. METHODS Retinal IR was induced in Ngb(More)
PURPOSE During the early phase of experimental autoimmune uveitis (EAU), before macrophages infiltrate the retina and uvea, photoreceptor mitochondrial oxidative stress, nitration of photoreceptor mitochondrial proteins, and release of cytochrome c have been observed. However, no apoptosis has been detected during this phase. In this study,(More)