Naoyuki Suguro

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The medaka (Oryzias latipes), a tiny fish, has been an excellent experimental model for molecular and developmental genetics, and is expected for being a "vertebrate" model animal for population genetics, because 1) there is abundant within-species variation, and 2) the whole genome sequence has been determined for one of the inbred strains. In spite of its(More)
We examined the genetic population structure of Lefua echigonia (Japanese name, hotoke-dojo) using polymerase chain reaction restriction-fragment length polymorphisms (PCR-RFLPs) of the mitochondrial cytochrome b gene and two allozymic loci. The phylogenetic relationships of L. echigonia and those among L. echigonia, Lefua sp. (nagare hotoke-dojo), and L.(More)
A facile, sensitive radioimmunoassay of estradiol 3-sulfate in pregnancy plasma was established. Highly specific antisera were raised in male rabbits against a conjugate of 6-oxoestradiol 3-sulfate-(O-carboxymethyl)oxime with bovine serum albumin. After purification of the antiserum, we coupled the crude globulin fraction to CNBr-activated Sepharose-4B and(More)
Antisera were prepared against two types of estradiol-3-sulfate-bovine serum albumin (BSA) conjugates. The haptens were coupled to BSA through the C-6 position in the steroid molecule by the glutaraldehyde (A) or the carbodiimide method (B). In comparison the antiserum produced by method A had a high affinity for estradiol-3-sulfate (Ka = 5.64 X 10(8) M-1);(More)
The Tokyo bitterling Tanakia tanago (Cyprinidae) was once found throughout the Kanto Plain, central Japan, but most of their habitats have been lost due to human activities such as urbanization and improvement of paddy fields. Subsequently, conservation efforts, including captive breeding and reintroduction, have been ongoing. However, the genetic(More)
田村 俊吉*・富沢 摂 夫 ・堤 璋二*・勝 呂 信雄 ・木津 弘司 ・伊藤 博夫 ・中井 一仁 ・北 進一 ・増田 紀男 Present address : * Department of Pharmacology , Tokyo Dental College, Chiyoda-ku, Tokyo. Concerning the cause of fatigue, there have been many assumptions on the basis of energy accumulation of substances inducing fatigue. There have been a lot of studies (1, 2) on piling up of the so-called(More)
Mechanisms that determine the strength of trophic cascades from fish to benthic algae via algivorous invertebrates in stream communities have not been clarified. Using seven fish species, we tested the hypothesis that the interspecific variation of predatory behavior of fishes affects the strength of trophic cascades in experimental streams. One or two(More)
Changes of gastric movements and ulcerogenic mechanisms in reserpine (6 mg/kg s.c.)-induced ulcer were studied in Wistar male rats weighing 180 to 220 g after fasting for about 20 hr. Under anesthesia with Nembutal (30 mg/kg i.p.), rats were fixed on their backs. The abdomen was incised and the stomach was exposed. Gastric movements of the fundic glandular(More)
Antisera were raised in male guinea pigs against 6-oxoestriol 3-sulfate O-carboxymethyloxime-bovine serum albumin (BSA) conjugate. The antisera to this antigen exhibited high affinity (Ka=4.7 X 10(9)M-1) and excellent specificity for estriol 3-sulfate, showing slight cross-reactions (less than 0.43%) with other estrogen sulfates, and no cross-reactivities(More)