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The present study describes the seasonal changes in lipid components of two brown algae, Sargassum horneri (Turner) and Cystoseira hakodatensis (Yendo), with specific reference to fucoxanthin (Fx) and n-3 (omega-3) polyunsaturated fatty acids (PUFAs). Young thalli of S. horneri were collected from different places, Nesaki and Matsushima, and cultivated in(More)
Sodium pumps (EC, Na+-ATPase), which mediate excretion of Na+ from the cell, play a crucial role in Na+ homeostasis in eukaryotic cells. The objective of this study is to understand the Na+ efflux system in a marine red alga. We identified a novel sodium pump gene, PyKPA2, from the marine red alga Porphyra yezoensis. The amino acid sequence of(More)
The polarized distribution of F-actin is important in providing the driving force for directional migration in mammalian leukocytes and Dictyostelium cells, in which compartmentation of phosphatidylinositol 3-kinase (PI3K) and phosphatidylinositol phosphatase is critical for the establishment of cell polarity. Since monospores from the red alga Porphyra(More)
Marine macroalgae play an important role in marine coastal ecosystems and are widely used as sea vegetation foodstuffs and for industrial purposes. Therefore, there have been increased demands for useful species and varieties of these macroalgae. However, genetic transformation in macroalgae has not yet been established. We have developed a dominant(More)
The life cycle of plants entails an alternation of generations, the diploid sporophyte and haploid gametophyte stages. There is little information about the characteristics of gene expression during each phase of marine macroalgae. Promoter analysis is a useful method for understanding transcriptional regulation; however, there is no report of promoter(More)
Polarity is a fundamental cell property essential for differentiation, proliferation and morphogenesis in unicellular and multicellular organisms. We have recently demonstrated that phosphatidylinositol 3-kinase (PI3K) activity is required for the establishment of anterior-posterior axis, leading to asymmetrical localization of F-actin in migrating(More)
Multiprotein bridging factor 1 (MBF1) is a highly conserved transcriptional co-activator involved in the regulation of diverse processes, such as environmental stress responses. We recently identified a novel MBF1 gene, PyMBF1, from the marine red alga Pyropia yezoensis. In this study, quantitative real-time PCR analysis revealed that PyMBF1 transcripts(More)
Na+/H+ antiporters are known to play a crucial role in pH and Na+ homeostasis. In the present study, we characterized the molecular structures and expression patterns of two Na+/H+ antiporters from the marine red alga Porphyra yezoensis (designated PySOS1 and PyNhaD). The full-length cDNAs of PySOS1 and PyNhaD were 5122 and 1804 bp, and contained open(More)
Abbreviations: AmCFP: humanized cyan fluorescent protein from Anemonia majano AMX: emzyme mix containing β-agarase, β-1,4-mannanase and β-1,3-xylanase ESL: enriched sealife GUS: β-glucuronidase MES: 2-(N-Morpholino)ethanesulfonic acid PH: Pleckstrin homology PyAct1: P. yezoensis actin1 PyGUS: P. yezoensis-adapted β-glucuronidase Despite the recent progress(More)
The asymmetrical distribution of F-actin directed by cell polarity has been observed during the migration of monospores from the red alga Porphyra yezoensis. The significance of Ca 2+ influx and phosphoinositide signalling during the formation of cell polarity in migrating monospores was analysed pharmacologically. The results indicate that the inhibition(More)