Naonori Sugai

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We have made several improvements in the method of fixation of the inner ear and the enzyme-histo-chemical technique for carbonic anhydrase (CA) detection. The results confirmed that CA is localized in the hair cells of the organ of Corti, Deiters' cells or nerve endings, inner pillar cells, Boettcher's cells, stria vascularis, spiral ligament, spiral(More)
Although carbonic anhydrase (CA) plays an important role in respiration, there is little information about CA in the respiratory system except for the lung. We examined the entire nasal cavity of guinea pig to identify CA activity using an enzyme-histochemical method. CA activity was detected in a group of morphologically distinct nasal epithelial cells.(More)
The effects of adding midazolam and bupivacaine to human cerebrospinal fluid in glass test tubes were examined by looking for changes in pH and a reduction in the transparency of the solution. Midazolam (n = 6), 0.25% bupivacaine (n = 6), 5 mg of midazolam in 6 mL of 0.25% bupivacaine (n = 6) and 5 mg of midazolam in 10 mL of saline (n = 6) were added to(More)
We immunohistochemically examined the distribution of nerve fibers among supporting cells of the cochlea by using the bloc-surface preparation. The existence of these nerve fibers was not very clear in the standard avidin-biotin complex (ABC) method. However, the standard ABC method complemented with silver intensification procedure provided very fine(More)
The tetanic force development of the human adductor pollicis muscle was studied under light anesthesia with nitrous oxide, oxygen, and Demerol, by the use of tetanic stimulation of the ulnar nerve at frequencies ranging from 10 to 100 Hz. The time necessary for the tetanic contraction to reach a plateau was longest at frequencies between 15 and 20 Hz.(More)
Intravenous dose-response relationships were used to correlate neuromuscular paralysis with the effects of fazadinium (AH 8165) on autonomic mechanisms in anaesthetized cats and rhesus monkeys and with cardiovascular effects in man. In cats and monkeys neuromuscular paralysis of the twitch responses of the gastrocnemius muscle by fazadinium was accompanied(More)
We examined the difference in the duration of action of vecuronium injected through a venous line infused with propofol and the duration of vecuronium infused into another venous line without propofol, in order to investigate the interaction between vecuronium and propofol within the intravenous lines. The subjects of the study are 8 patients, (ASA grade 1(More)
Localization of carbonic anhydrase (CA) was investigated in the rat eye by light and electron microscopy, and the inhibitory effects on CA distribution in the rat eye were compared between dorzolamide and acetazolamide. The cobalt sulfide histochemical method was used to study the CA localization. Acidic osmification was carried out for postfixation of the(More)
Carbonic anhydrase (CA) is distributed in a wide variety of animal cells and tissues, especially in cells and tissues involved in rapid exchange or transport of protons, bicarbonate, or other ions. Although localization of this enzyme and its physiological roles in the inner ear have been investigated extensively, full clarification has yet to be achieved.(More)
From Sheiner's equation on pharmacokinetics and pharmacodynamics, we derived a new equation which described the pharmacodynamics of nondepolarizing muscle relaxants during the onset phase. This equation showed that log (l0/l-1) had a linear relation to log (t) where "t" is the time after the administration of nondepolarizing muscle relaxant and "l" and "l0"(More)