Learn More
PURPOSE To establish a rabbit dry eye model with topical medication of the ocular preparation preservative benzalkonium chloride (BAC). METHODS Sixteen white rabbits were used. One eye of each rabbit was chosen randomly for topical administration of 0.1% BAC twice daily for 14 days. The other untreated eyes served as controls. Schirmer test, fluorescein,(More)
This study was to develop a method using phospholipase A(2) (PLA(2)) to prepare acellular porcine corneal stroma (APCS) for tissue engineering. The APCS was prepared from native porcine cornea (NPC) that was treated with 200 U/ml PLA(2) and 0.5% sodium deoxycholate (SD). The removal of DNA content, representing decellularization efficiency, reached to 91%,(More)
BACKGROUND Dry eye is a common disease worldwide, and animal models are critical for the study of it. At present, there is no research about the stability of the extant animal models, which may have negative implications for previous dry eye studies. In this study, we observed the stability of a rabbit dry eye model induced by the topical benzalkonium(More)
To investigate the feasibility of using acellular porcine limbal stroma for limbal stem cell microenvironment reconstruction. Limbal reconstruction was performed in rabbit partial limbal defect models. Rabbits were randomly divided into four groups: acellular porcine limbal stroma, de-epithelized rabbit limbal autograft stroma, de-epithelized porcine limbal(More)
PURPOSE Tectonic lamellar keratoplasty (TLKP) is a primary surgical procedure to improve the condition of the recipient bed in high-risk corneal transplantation. It is usually performed for a secondary optical penetrating keratoplasty (PKP). The present study was undertaken to explore a new strategy for TLKP using acellular corneal stroma (ACS) prepared by(More)
PURPOSE The objective of this study was to produce a porcine corneal acellular matrix (ACM) and assess its possibility for biomedical applications. METHODS Porcine corneas were treated with various concentrations of sodium dodecylsulfate for different lengths of time. Optimal conditions for processing the ACM were noted regarding removal of all cellular(More)
Canalicular laceration, especially lower canaliculus laceration, is the most common type of trauma of the lacrimal system. The primary aim of management of these cases is to restore the canalicular system both anatomically and functionally. Previously, we have successfully developed a modified soft probe to treat lacrimal passage obstruction. 2 Herein, we(More)
The objective of this study was to explore the potential role of human telomerase reverse transcriptase (TERT) in extending the proliferative lifespan of human corneal endothelial cells (HCECs) under long-term cultivation. A primary culture was initiated with a pure population of HCECs in DMEM/F12 media containing 10% fetal bovine serum and other various(More)
BACKGROUND Valsalva retinopathy is a rare but sight-threatening condition with sudden visual loss. Twin-pregnancy is known as a confirmed risk factor for Valsalva retinopathy. CASE REPORT A 21-year-old twin-pregnant Chinese woman with 31 weeks of gestation complained of a sudden decrease of visual acuity in her right eye for five hours. Based on the(More)