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The direct voltammetry and electrocatalytic properties of catalase (Cat) in polyacrylamide (PAM) hydrogel films cast on pyrolytic graphite (PG) electrodes were investigated. Cat-PAM film electrodes showed a pair of well-defined and nearly reversible cyclic voltammetry peaks for Cat Fe(III)/Fe(II) redox couples at approximately -0.46 V vs. SCE in pH 7.0(More)
A novel thin film of heme proteins, including hemoglobin (Hb), myoglobin (Mb), and catalase (Cat), was successfully assembled layer by layer with polyamidoamine (PAMAM) dendrimers on different solid surfaces. At pH 7.0, protonated PAMAM possesses positive surface charges, whereas the proteins have net negative surface charges at pH above their isoelectric(More)
Electrochemical behavior of myoglobin (Mb) incorporated in polyacrylamide (PAM) hydrogel films cast on pyrolytic graphite (PG) electrodes were investigated. Mb-PAM film electrodes showed a pair of well-defined and nearly reversible cyclic voltammetric peaks for Mb Fe(III)/Fe(II) redox couple at about -0.27 (vs. SCE) in pH 5.5 buffers. The electron exchange(More)
Protein-chitosan (CS) films were made by casting a solution of proteins and CS on pyrolytic graphite electrodes. Myoglobin (Mb), hemoglobin (Hb), and horseradish peroxidase (HRP) incorporated in CS films gave a pair of stable, well-defined, and quasi-reversible cyclic voltammetric peaks at about -0.33V vs saturated calomel electrode in pH 7 buffers,(More)
A novel core-shell protein nanocluster film, designated as clay-(Hb/PSS)(n), was fabricated on pyrolytic graphite (PG) electrodes. Positively charged hemoglobin (Hb) at pH 5.5 and negatively charged poly(styrenesulfonate) (PSS) were first assembled layer by layer on surface of clay nanoparticles from their solutions mainly by electrostatic attraction,(More)
Anionic surfactant dihexadecyl phosphate (DHP) with two hydrocarbon chains can be self-assembled into a double-layer structure with tail-to-tail configuration and negatively charged head groups toward outside in its aqueous dispersion. Due to this unique biomembrane-like structure, the "charge reversal" in DHP adsorption on solid surface was realized, and(More)
Stable layer-by-layer electroactive films were grown on pyrolytic graphite (PG) electrodes by alternate adsorption of layers of polyanionic poly(vinyl sulfonate) (PVS) and positively charged hemoglobin (Hb) from their aqueous solutions. Cyclic voltammetry (CV) of [PVS/Hb]n films showed a pair of well-defined and nearly reversible peaks at about - 0.28 V vs.(More)
Myoglobin (Mb), with different net surface charges at different pH in buffers and negatively charged hyaluronic acid (HA) at pH 5.0 in solutions were alternately adsorbed onto various solid surfaces and successfully assembled into {Mb/HA}(n) layer-by-layer films. The Mb in {Mb/HA}(n) films showed a quasi-reversible cyclic voltammetry (CV) response for its(More)
Biomembrane-like polyionic complex, 2C(12)N(+)PA(-), was prepared by reacting sodium polyacrylate (Na(+)PA(-)) with didodecyldimethylammonium bromide (2C(12)N(+)Br(-)). Stable thin films made from 2C(12)N(+)PA(-), with incorporated myoglobin (Mb), on pyrolytic graphite (PG) electrodes were then characterized by electrochemistry and other techniques. Cyclic(More)
In the present work, a new kind of myoglobin (Mb)/Au nanoparticles composite film was fabricated on pyrolytic graphite (PG) electrodes. Oppositely charged chitosan (CS) and silica (SiO(2)) nanoparticles were alternately adsorbed on the PG surface by the electrostatic interaction between them, forming {CS/SiO(2)}(5) layer-by-layer films. Mb and HAuCl(4) in(More)