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Precise control of gene expression is critical for embryo development in both animals and plants. We report that Arabidopsis thaliana GLUTAMINE-RICH PROTEIN23 (GRP23) is a pentatricopeptide repeat (PPR) protein that functions as a potential regulator of gene expression during early embryogenesis in Arabidopsis. Loss-of-function mutations of GRP23 caused the(More)
RNA biogenesis is essential and vital for accurate expression of genes. It is obvious that cells cannot continue normal metabolism when RNA splicing is interfered with. sgt13018 is such a mutant, with partial loss of function of GAMETOPHYTIC FACTOR 1 (GFA1); a gene likely involved in RNA biogenesis in Arabidopsis. The mutant is featured in the phenotype of(More)
In flowering plants, gametogenesis generates multicellular male and female gametophytes. In the model system Arabidopsis, the male gametophyte or pollen grain contains two sperm cells and a vegetative cell. The female gametophyte or embryo sac contains seven cells, namely one egg, two synergids, one central cell and three antipodal cells. Double(More)
Antibodies targeting T cells and B cells are increasingly used for immunosuppression in clinical transplantation. However, the impact of T-cell depletion by antibodies on B-cell homeostasis is poorly understood. Using a mouse model of allosensitization with skin allograft, we investigated whether targeting T cells by anti-CD3ε alters peripheral B-cell(More)
As a model system for efficient analysis of plant gene function, Arabidopsis can easily be transformed by Agrobacterium-mediated gene transfer. Among the various transformation methods developed during the past two decades, the vacuum infiltration transformation and the floral dip method are most widely used. Compared with conventional plant transformation(More)
To perform an effective genetic screen for embryo-specific mutants is a prerequisite for understanding the molecular mechanisms that control Arabidopsis embryo development. Mutagenesis based on either T-DNA or transposon insertion has been successfully used in identifying embryonic mutants. We present here a typical genetic screen for putative embryonic(More)
Precise control of gene expression is critical for embryo development in both animals and plants. We report that Arabidopsis thaliana GLUTAMINE-RICH PROTEIN23 (GRP23) is a pentatricopeptide repeat (PPR) protein that functions as a potential regulator of gene expression during early embryogenesis in Arabidopsis. Loss-of-function mutations of GRP23 caused the(More)
This paper presents a predictive model of solid oxide fuel cell (SOFC) stacks for thermal management by using a support vector machine (SVM). The operating temperature of the SOFC stack is the most important variable controlled for the generation system. To carry out the control research on the stack thermal management, the predictive model of the stack(More)
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