Nae-Hwey Miao

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By applying pre-treatment with high concentrations of sucrose and glycerol prior to desiccation and subsequent freezing in liquid nitrogen, successful cryopreservation with high recovery rate was achieved in Oncidium PLB (protocorm-like body). Cellular and subcellular changes after each step in various cryopreservation regimes were examined to elucidate the(More)
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