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BACKGROUND Whether or not cytokine secretion is impaired in patients with small-cell lung cancer (SCLC), is unknown. We therefore investigated whether cytokine secretion by immunocompetent cells may be suppressed in patients with SCLC. PATIENTS AND METHODS We determined cytokine secretion by lymphocytes and monocytes in whole blood cell cultures from 58(More)
Viral hemorrhagic fevers are characterized by enhanced permeability. One of the most affected target organs of hantavirus-induced hemorrhagic fever with renal syndrome is the kidney, and an infection often results in acute renal failure. To study the underlying cellular effects leading to kidney dysfunction, we infected human renal cell types in vitro that(More)
In recent years, metabolomics developed to an accepted and valuable tool in life sciences. Substantial improvements of analytical hardware allow metabolomics to run routinely now. Data are successfully used to investigate genotype-phenotype relations of strains and mutants. Metabolomics facilitates metabolic engineering to optimise mircoorganisms for white(More)
Several non-animal methods are now available to address the key events leading to skin sensitization as defined by the adverse outcome pathway. The KeratinoSens assay addresses the cellular event of keratinocyte activation and is a method accepted under OECD TG 442D. In this study, the results of an inter-laboratory evaluation of the "me-too" LuSens assay,(More)
A method has been developed for quantification of 20 amino acids as well as 13 (15)N-labeled amino acids in barley plants. The amino acids were extracted from plant tissues using aqueous HCl-ethanol and directly analyzed without further purification. Analysis of the underivatized amino acids was performed by liquid chromatography (LC)-electrospray(More)
In this chapter, we describe a method for quantification of 20 proteinogenic amino acids as well as 13 (15)N-labeled amino acids by liquid chromatography-mass spectrometry without the need for derivatization and use of organic solvents. Analysis of the underivatized amino acids is performed by liquid chromatography-electrospray ionization-tandem mass(More)
Stock number: BGS 401 Locus name: Eceriferum-f Locus symbol: cer-f Previous nomenclature and gene symbolization: None. Inheritance: Monofactorial recessive (3). Located in chromosome 1H (1); associated with SNP markers 1_0764 to 2_1126 (positions 61.51 to 110.10) in 1H of the Bowman backcrossed-derived line BW110 (1). Previously located in chromosome 7H(More)