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Morphine and several other opioids are important drugs for the treatment of acute and chronic pain. Opioid-induced analgesia is predominantly mediated by the μ-opioid receptor (MOR). When administered to humans, complex metabolic pathways lead to generation of many metabolites, nine of which may be considered major metabolites. While the properties of the(More)
Cell-free expression is emerging as a prime method for the rapid production of preparative quantities of high-quality membrane protein samples. The technology facilitates easy access to large numbers of proteins that have been extremely difficult to obtain. Most frequently used are cell-free systems based on extracts of Escherichia coli cells, and the(More)
Allosteric modulators have been identified for several G protein-coupled receptors, most notably muscarinic receptors. To study their mechanism of action, we made use of a recently developed technique to generate fluorescence resonance energy transfer (FRET)-based sensors to monitor G protein-coupled receptor activation. Cyan fluorescent protein was fused(More)
The immune response in higher vertebrates is a complex phenomenon mediated by a network of cellular interactions. Major histocompatibility complex (MHC) class II DR, DQ, and DP are highly polymorphic cell surface glycoproteins which play a central role in immunity by binding and presenting peptides to helper T lymphocytes (Brown et al. 1993). These(More)
An additional HLA-DRB1*16 allele was identified using XIth International Histocompatibility Workshop polymerase chain reaction sequence-specific oligoprobe (PCRSSO) hybridization after the PCR typing protocol. This allele (DRB1*1608) was found in two unrelated Caucasian patients. After HLA-DRB1 second exon generic amplification using the primers described(More)
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