Nadine C Milos

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The early heart anlagen of Xenopus laevis embryos were exposed to purified embryonic galactoside-binding lectin or its potent hapten inhibitor thiodigalactoside (TDG). Heart development was then studied using a variety of microscopical techniques. Conotruncal morphology and positioning with respect to the ventricle are altered in treated animals. In 34% of(More)
Extracts obtained from chick embryos at the pre-gastrula and gastrula stages are able to agglutinate trypsinized rabbit erythrocytes fixed with glutaraldehyde. Agglutination is inhibited by saccharides sharing a beta-D-galactopyranoside configuration. Agglutinin activity is also inhibited by desialysed fetuin which bears terminal galactose residues but not(More)
Cranial neural crest of Xenopus laevis at different stages of development from neurulation to metamorphosis was studied for the expression of the endogenous galactoside-binding lectin of Xenopus using immunocytochemistry. The presence and localization of members of the N-CAM and cadherin cell adhesion families were also investigated. Lectin and the other(More)
A monoclonal antibody that we have produced against the neural crest-stage galactoside-binding lectin of Xenopus laevis has been used to confront neural crest cells in Xenopus embryos in vivo. Confronted tadpoles have been raised to stage 47 (premetamorphosis) and examined for jaw defects in whole mounts and tissue sections. Antibody treatment correlates(More)
The regions of the developing craniofacial skeleton and gut of Xenopus laevis have been confronted in vivo with purified embryonic galactoside-binding lectin or its hapten inhibitor thiodigalactoside (TDG). Confrontation was carried out at stage 24-26 (cranial neural crest migrating). Further development of the head skeleton and gut has been monitored in(More)
Control charts offer a way for healthcare organizations to react appropriately to unexpected changes in performance indicators. Control charts demonstrate whether an immediate remedy or a redesign of a process is needed. An understanding of variation in processes can help in comprehending performance and avoiding identification of data trends that do not(More)
Cells from the extraembryonic endoderm of the gastrulating chick embryo contain a β-D-galactoside-binding lectin inhibited by thiodigalactoside (TDG). When cell suspensions are cultured in stationary culture in the presence of exogenously added purified blastoderm lectin or TDG, their attachment to the substratum is delayed and decreased compared to(More)
Odontoblasts are secretory cells displaying epithelial and mesenchymal features, which exist in a monolayer at the interface between the dentin and pulp of a tooth. During embryogenesis, these cells form a dentin shell and throughout life continue to produce dentin while, also acting as sensor cells helping to mediate tooth sensitivity. In this process,(More)