Nadia I Tarasova

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The mechanism by which vasoactive intestinal peptide (VIP)-ellipticine (E) conjugates are cytotoxic for human lung cancer cells was investigated. VIP-alanyl-leucyl-alanyl-leucyl-alanine (ALALA)-E and VIP-leucyl-alanyl-leucyl-alanine (LALA)-E inhibited (125)I-VIP binding to NCI-H1299 cells with an IC50 values of 0.5 and 0.1 microM, respectively. VIP-ALALA-E(More)
The crystal and molecular structures of human progastricsin (hPGC) have been determined using multiple isomorphous replacement methods and anomalous scattering in conjunction with a phased translation function. The structure has been refined to a conventional R-factor (= sigma parallel Fo magnitude of - magnitude of Fc parallel / sigma magnitude of Fo(More)
The three-dimensional crystal structure of human pepsin and that of its complex with pepstatin have been solved by X-ray crystallographic methods. The native pepsin structure has been refined with data collected to 2.2 A resolution to an R-factor of 19.7%. The pepsin:pepstatin structure has been refined with data to 2.0 A resolution to an R-factor of 18.5%.(More)
The effect of picolinic acid (PA) on SPEV cell proliferation is found to be different from that on normal and virus transformed NRC cells, and on spontaneously transformed CHO cells. It is shown that SPEV cells are arrested by PA at the end of G1-phase and at the beginning of S-phase and probably in G2-phase of the cell cycle. Ferrous ions remove the G1/S(More)
Chymotrypsinhydrolyzes the p-nitroanilide of pyroglutamyl-L-phenylalanine with the splitting out of p-nitroaniline, which is determined spectrophotometrically from its absorption at 410 nm. The pyroglutamic acid residue increases the solubility of this substrate in water as compared with the p-nitroanilide of N-(3-carboxypropionyl)-L-phenylalanine that is(More)
The authors present the results of clinico-laboratory examination of 30 patients with a congenital (hereditary) hemorrhagic tendency associated with an isolated disturbance of the adhesive-aggregation platelet function. Several variants of disaggregational thrombocytopathies were distinguished. Characteristics of hemorrhagic phenomena in the patients is(More)
Clotting of casein provides a sensitive method for detection of proteases after gel electrophoresis. The method is here designated "caseogram." After electrophoresis the gel was equilibrated with 0.15-0.3 M sodium acetate, pH 5.3, and an 1% agarose gel containing 1% skim-milk powder in 0.1 M sodium acetate, pH 5.3, was placed on top of the electrophoresis(More)
Possibility of long-term existence of closed model membrane structures loaded with low molecular compounds of bivalent ferrum was shown. Liposomes prepared from phospholipids of egg yolk were used as a model. There were found similar effects of heating and detergents on the formation of nitrosyl complexes of non-heme iron (complexes 2.03) in the model(More)