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Proper organization of microtubule arrays is essential for intracellular trafficking and cell motility. It is generally assumed that most if not all microtubules in vertebrate somatic cells are formed by the centrosome. Here we demonstrate that a large number of microtubules in untreated human cells originate from the Golgi apparatus in a(More)
Microtubules are indispensable for Golgi complex assembly and maintenance, which are integral parts of cytoplasm organization during interphase in mammalian cells. Here, we show that two discrete microtubule subsets drive two distinct, yet simultaneous, stages of Golgi assembly. In addition to the radial centrosomal microtubule array, which positions the(More)
The RNA polymerase II (Pol II)-type mechanism is conserved from yeast to human. After transcription initiation, Pol II usually pauses upstream of or in a nucleosome within the early transcribed region of a gene. Then Pol II overcomes the initial nucleosomal barrier and efficiently proceeds through chromatin. At a low to moderate transcription rate, Pol II(More)
The kinesin KIF1C is known to regulate podosomes, actin-rich adhesion structures that remodel the extracellular matrix during physiological processes. Here, we show that KIF1C is a player in the podosome-inducing signaling cascade. Upon induction of podosome formation by protein kinase C (PKC), KIF1C translocation to the cell periphery intensifies and KIF1C(More)
The tumor suppressor and microtubule-associated protein Ras association domain family 1A (RASSF1A) has a major effect on many cellular processes, such as cell cycle progression and apoptosis. RASSF1A expression is frequently silenced in cancer and is associated with increased metastasis. Therefore we tested the hypothesis that RASSF1A regulates microtubule(More)
Nucleosome forms a so-called nucleosome barrier, which is a serious difficulty for a transcriptional apparatus. We considered studies of formation and nucleosome barrier closing by some types of RNA polymerases. We showed that different polymerases use similar mechanisms for chromatin transcription. We present data testifying to the high similarity between(More)
Chromatin provides for the dense packing of DNA in eukaryotic cell nuclei and its proper functioning. The present review describes mechanisms of chromatin transcription by RNA polymerase III (RNAP III). This mechanism is characteristic of eukaryotic RNAP III, some bacteriophage RNAPs, and many ATP-dependent chromatin remodeling complexes. During the(More)
The structures of some cryptogenes of previously unstudied species of homoxenous trypanosomatids that belong to different phylogenetic groups has been analyzed. New examples of the editing domain length reduction for A6 and COIII were found. A comparative analysis of the sequences allowed us to divide the cryptogenes into three groups (patterns) according(More)
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