Nadezhda V Tsymbalenko

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There is an emerging link between copper metabolism, tumor growth and efficiency of antitumor treatment with platinum drugs or copper chelators. So there is an urgent need for well-defined and reproduced animal models with different states of copper metabolism. In the present study an animal model (rats and mice) with switching copper status in blood serum(More)
Immunoblotting has been used to detect ceruloplasmin (Cp) polypeptides in rat mitochondria isolated from the brain, liver, testes, and mammary glands. It has been found that Cp is contained in the matrix and inner membranes of mitochondria, and that its molecular weight corresponds to the nonglycosylated form of the protein. Computer analysis has not(More)
This research focuses on the role of milk ceruloplasmin (Cp), the main extracellular copper-containing protein of vertebrates, as a source of copper for newborns. In the first part of the study, Cp concentration and Cp-associated copper were measured in human skimmed milk at the 1st and the 5th days postpartum. It was shown that most of the copper was(More)
The influence of short and prolonged diet containing silver ions (Ag-diet) on copper metabolism was studied. Two groups of animals were used: one group of adult rats received a Ag-diet for one month (Ag-A1) and another group received a Ag-diet for 6 months from birth (Ag-N6). In Ag-A1 rats, the Ag-diet caused a dramatic decrease of copper status indexes(More)
Some peculiarities of rat milk ceruloplasmin (CP) biosynthesis have been analysed. Electrophoretic and immunoelectrophoretic data suggest that rat milk contains up to 20 mg/100 ml of CP. Rat milk CP is represented by a single molecular form whose molecular mass, enzymatic activity and antigenic properties are fully identical with those of serumal CP.(More)
ANNOTATION: Translation in all open reading frames (ORF) of human ceruloplasmin (Cp) pseudogene revealed the only translating sequence of 984 nucleotides. The amino acid sequence contains a signal peptide for mitochondrial protein import at N-terminus. The predicted protein without taking the signal peptide into consideration has 92% identity to the(More)
The putative product of CTR1 (SLC31A1 according to the Entrez data base) is regarded as the main candidate for an eukaryotic transmembrane copper importer. The tissue-specific function of mammalian CTR1 is still unknown. A quasi-steady-state level of the CTR1 mRNA was assayed by semiquantitative RT-PCR and compared with the copper status in rat organs(More)
The content of ceruloplasmin (Cp) was determined in 96 samples of human breast milk using rocket immunoelectrophoresis and measurement of Cp oxidase activity. The concentration of immunoreactive Cp in milk decreased about 9 times during the first 20 days of lactation while the specific oxidase activity decreased only 4 times. Two-dimensional electrophoresis(More)
24 Recent data has clearly demonstrated that CTR1, a plasma membrane protein belonging to the family of high-affinity copper importers, is involved in the transport of cisplatin, an extensively used antineoplastic drug, into cells [1]. However, it is hardly probable that the mechanism of Pt(II) binding and transport into the cell is identical to that of Cu(More)
One of the key mammalian proteins involved in carrying iron across cell membranes and transport of extracellular copper is ceruloplasmin (Cp, EC 1.16.3.1), a copper-containing glycoprotein that is synthesized predominantly in the liver and then secreted to the blood stream. In addition to this molecular form of Cp, other tissue-specific soluble Cps are(More)