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The far-red fluorescent protein mKate (lambda(ex), 588 nm; lambda(em), 635 nm; chromophore-forming triad Met(63)-Tyr(64)-Gly(65)), originating from wild-type red fluorescent progenitor eqFP578 (sea anemone Entacmaea quadricolor), is monomeric and characterized by the pronounced pH dependence of fluorescence, relatively high brightness, and high(More)
The three-dimensional structures of the wild-type red (zRFP574) and green (zGFP506) fluorescent proteins (FP) from the button polyp Zoanthus have been determined at 1.51 and 2.2 A resolution, respectively. In addition, the crystal structures of a zGFP506 variant (zGFP506_N66D) with replacement of the first chromophore-forming residue (Asn66 to Asp) have(More)
The far-red fluorescent protein mKate (␭ ex , 588 nm; ␭ em , 635 nm; chromophore-forming triad Met 63-Tyr 64-Gly 65), originating from wild-type red fluorescent progenitor eqFP578 (sea anemone Entacmaea quadricolor), is monomeric and characterized by the pronounced pH dependence of fluorescence, relatively high brightness, and high photostability. The(More)
  • N Pletneva, V Pletnev, T Tikhonova, A A Pakhomov, V Popov, V I Martynov +9 others
  • 2007
BE5092 Refined crystal structures of red and green fluorescent proteins from the button polyp Zoanthus Pletneva et al. Synopsis The crystal structure of the red fluorescent protein zRFP574 from Zoanthus sp. 2 at 1.51 A ˚ resolution allowed a detailed description of the chromophore derived from decarboxylation of the aspartate side chain within the(More)
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