Learn More
We cloned complementary DNA (cDNA) encoding aquaporin 1 (AQP1) and arginine vasotocin receptor (AVT-R) from gill and kidney tissue of the black porgy (Acanthopagrus schlegeli), respectively. Black porgy AQP1 cDNA consists of 786 base pairs (bp) and encodes a protein of 261 amino acids, and AVT-R partial cDNA consists of 606 bp. To investigate the(More)
We determined oxidative stress by measuring the expression and activity of 3 antioxidant enzymes [Cu/Zn-superoxide dismutase (Cu/Zn-SOD), catalase (CAT) and glutathione peroxidase (GPX)] in black porgy exposed to thermal (20 degrees C-->30 degrees C) and hypoosmotic (35 psu-->10 psu and 0 psu) stresses. The expression and activity of antioxidant enzymes(More)
The present study aimed to test starvation-induced oxidative stress in the cinnamon clownfish Amphiprion melanopus illuminated by light-emitting diodes (LEDs): red (peak at 630 nm), green (peak at 530 nm), and blue (peak at 450 nm) within a visible light. We investigated the oxidative stress induced by starvation for 12 days during illumination with 3 LED(More)
We tested the mitigating effects of melatonin injections or irradiation from green-wavelength light-emitting diodes (LEDs) on goldfish (Carassius auratus) exposed to thermal stress (high water temperature, 30 °C). The effects of the two treatments were assessed by measuring the expression and activity levels of the antioxidant enzymes, superoxide dismutase(More)
Gonadotropin-releasing hormones (GnRHs) play pivotal roles in the control of reproduction and gonadal maturation in teleost fish. Fish have multiple GnRH genes that encode structurally distinct peptides. We identified salmon GnRH (sGnRH), seabream GnRH (sbGnRH), and chicken GnRH-II (cGnRH-II) by cDNA cloning in cinnamon clownfish (Amphiprion melanopus)(More)
The caspase-3 appears to be a key protease in the apoptotic pathway. We identified caspase-3 complementary DNAs from the ovaries of the protandrous cinnamon clownfish (Amphiprion melanopus), and investigated its mRNA and proteins, and activity levels during the sex change (I, mature male; II, male at 90 days after removing of the female; and III, mature(More)
We investigated the effects of the different light emitting diodes (LEDs; red, green, blue, and mixed purple) on oxidative stress by measuring the expression and activities of antioxidant enzymes, plasma H2O2, lipid peroxidation (LPO), aspartate aminotransferase (AspAT), alanine aminotransferase (AlaAT) levels, and melatonin during thermal stress (low-water(More)
Selenium (Se) is an essential element that has relevant roles in growth, immunity, and the protection of cell membranes in organisms under normal conditions. The aim of this study was to test the antioxidant and immune functions of goldfish Carassius auratus after Se treatment. At the end of the experiment, levels of antioxidant enzymes (superoxide(More)
In the present study, we investigated the expression pattern of estrogen receptors (esr) and vitellogenin (vtg) mRNA in the gonads and liver during sex change in cinnamon clownfish by using quantitative polymerase chain reaction. We divided gonadal development during the sex change from male to female into 3 stages (mature male, male at 90days after(More)
This study aimed to examine the role of 2 aquaporin (AQP) isoforms (AQP3, and -8) in sockeye salmon (Oncorhynchus nerka) in response to a hyperosmotic challenge from freshwater to seawater (SW) during the parr and smoltification (smolt) stages. AQP3 mRNA was primarily detected in the osmoregulatory organs, such as gills, while AQP8 mRNA was primarily found(More)