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Definition of the bacterial N‐glycosylation site consensus sequence
Bacterial N‐glycosylation site selection is more specific than the eukaryotic equivalent with respect to the polypeptide acceptor sequence. Expand
Structure of the N-Linked Glycan Present on Multiple Glycoproteins in the Gram-negative Bacterium, Campylobacter jejuni *
Comparison of thepgl locus with that of Neisseria meningitidissuggested that most of the homologous genes are probably involved in the biosynthesis of bacillosamine, and at least 22 glycoproteins were identified. Expand
The Genetic Bases for the Variation in the Lipo-oligosaccharide of the Mucosal Pathogen, Campylobacter jejuni
Compared the lipo-oligosaccharide biosynthesis loci from 11 Campylobacter jejunistrains expressing a total of 8 different ganglioside mimics in their LOS outer cores, at least five distinct mechanisms that allow C. jejuni to vary the structure of the LOSouter core are identified. Expand
Biosynthesis of Ganglioside Mimics in Campylobacter jejuni OH4384
We have applied two strategies for the cloning of four genes responsible for the biosynthesis of the GT1a ganglioside mimic in the lipooligosaccharide (LOS) of a bacterial pathogen,CampylobacterExpand
Functional Characterization of Dehydratase/Aminotransferase Pairs from Helicobacter and Campylobacter
Helicobacter pylori and Campylobacter jejuni have been shown to modify their flagellins with pseudaminic acid (Pse), via O-linkage, while C. jejuni also possesses a general protein glycosylationExpand
Quantitative Analysis of Bacterial Toxin Affinity and Specificity for Glycolipid Receptors by Surface Plasmon Resonance*
The primary virulence factors of many pathogenic bacteria are secreted protein toxins which bind to glycolipid receptors on host cell surfaces. The binding specificities of three such toxins forExpand
Structure of the Complex of Maclura pomiferaAgglutinin and the T-antigen Disaccharide, Galβ1,3GalNAc*
The minimum energy conformation expected for the disaccharide is retained, despite its mediating the tetramer-tetramer interactions in the crystal packing, and the resulting lattice is comparable to those seen for complexes of other lectins with branched glycopeptides. Expand
Cloning of the Lipooligosaccharide α-2,3-Sialyltransferase from the Bacterial Pathogens Neisseria meningitidis and Neisseria gonorrhoeae*
This study is the first example of the cloning, expression, and examination of α-2,3-sialyltransferase activity from a bacterial source. Expand
A Single Bifunctional UDP-GlcNAc/Glc 4-Epimerase Supports the Synthesis of Three Cell Surface Glycoconjugates in Campylobacter jejuni*
Data indicate that Gne is the enzyme providing Gal and GalNAc residues with the synthesis of all three cell-surface carbohydrates in C. jejuni Gne, a bifunctional UDP-GlcNAc/Glc 4-epimerase. Expand
Structural and Functional Characterization of PseC, an Aminotransferase Involved in the Biosynthesis of Pseudaminic Acid, an Essential Flagellar Modification in Helicobacter pylori*
Helicobacter pylori flagellin is heavily glycosylated with the novel sialic acid-like nonulosonate, pseudaminic acid (Pse). The glycosylation process is essential for assembly of functional flagellarExpand