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Effects of hydrostatic pressure on the ultrastructure and leakage of internal substances in the yeast Saccharomyces cerevisiae
- S. Shimada, Masayasu Andou, N. Naito, N. Yamada, M. Osumi, R. Hayashi
- Biology, ChemistryApplied Microbiology and Biotechnology
- 1 October 1993
The structural damage of pressure-treated cells was accompanied by the leakage of internal substances and amounts of individual metal ion release varied with the magnitude of hydrostatic pressures over 300 MPa, which suggests that the ions can be removed from the yeast cells separately by hydrostatic pressure treatment.
Three-dimensional structure of the prolamellar body in squash etioplasts
SummaryHigh resolution scanning electron microscopy revealed that the basic unit of the paracrystalline network in squash prolamellar body is a tetrapodal structure, which has four short tubular arms…
Cell wall formation in regenerating protoplasts of Schizosaccharomyces pombe: study by high resolution, low voltage scanning electron microscopy.
It is suggested that beta-glucan is the main component of the microfibrils and that it plays an important role in the formation of the cell wall in S. pombe.
Scanning Electron Microscopy on the Antheridium of Conocephalum Conicum
Actin is associated with the formation of the cell wall in reverting protoplasts of the fission yeast Schizosaccharomyces pombe.
It is demonstrated that actin is associated with initiation of cell wall formation, the proper deposition ofcell wall materials, and maintaining the normal morphology of reverting protoplasts.
Ultrahigh-resolution low-voltage SEM reveals ultrastructure of the glucan network formation from fission yeast protoplast.
- M. Osumi, N. Yamada, H. Yaguchi, H. Kobori, T. Nagatani, M. Sato
- EngineeringJournal of electron microscopy
- 1 August 1995
Improved ultrahigh-resolution low-voltage SEM (UHR-LVSEM) images of reverting protoplasts treated with glucanase confirmed that the particles scattered on the protoplast surface in the initial stage of regeneration were glucan in nature.
Three-dimensional structure of the crystalloid in the microbody of Kloeckera sp.: composite crystal model
- M. Osumi, M. Nagano, N. Yamada, J. Hosoi, M. Yanagida
- Physics, ChemistryJournal of bacteriology
- 1 July 1982
Electron microscopic investigations using the cryosectioning technique demonstrated that the crystalloid was composed of two different types of particles, large and small ones, arranged alternately and making up the composite crystal of rock salt structure.
High resolution, low voltage scanning electron microscopy of uncoated yeast cells fixed by the freeze-substitution method.
The effect of bifonazole on the structure of Trichophyton mentagrophytes.
The results strongly suggest that subinhibitory concentrations of bifonazole profoundly affected the normal growth and induced degenerative changes of the hyphae of T. mentagrophytes probably by affecting some essential metabolism or structure of the fungal cell.
Contrast Enhancement by Ruthenium Tetroxide for Observation of the Ultrastructure of Yeast Cells
To reveal the ultrastructure of regenerating protoplasts of Schizosacchgaromyces pombe, samples were treated with ruthenium tetroxide (RuO 4 ) after conventional fixation and then observed with a…