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Purification and properties of acyl-CoA oxidase from rat liver.
Acyl-CoA oxidase, the initial enzyme of the peroxisomal beta-oxidation system, was purified from rat liver. The final preparation was judged to be nearly homogeneous from the results of sedimentationExpand
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Properties of mitochondria and peroxisomal enoyl-CoA hydratases from rat liver.
Mitochondrial and peroxisomal enoyl-CoA hydratases were purified from rat liver. The mitochondrial enzyme, with a molecular weight of 161,000, was composed of 6 identical subunits. The molecularExpand
  • 126
  • 3
Properties of peroxisomal 3-ketoacyl-coA thiolase from rat liver.
Peroxisomal 3-ketoacyl-CoA thiolase has a molecular weight of 89,000 and consists of 2 polypeptide chains of identical size. The enzyme has no interchain disulfide bonds and is reversibly dissociatedExpand
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Peroxidase activity in thyroid gland and partial purification of the enzyme.
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CONFORMATIONAL STUDIES ON PROTEINS BY ISOELECTRIC FOCUSING *
  • N. Ui
  • Chemistry, Medicine
  • Annals of the New York Academy of Sciences
  • 1 June 1973
Most proteins, in their native state, are folded into characteristic three-dimensional structures. Generally, dissociable groups in proteins, both acidic and basic, are located on the outside ofExpand
  • 54
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Ion-exchange chromatographic analysis of iodothyronines.
Abstract An ion-exchange chromatographic procedure for the analysis of iodothyronine mixtures containing iodotyrosines is described. Eight different iodothyronines are separated into five peaks andExpand
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Isoelectric points and conformation of proteins. II. Isoelectric focusing of alpha-chymotrypsin and its inactive derivative.
  • N. Ui
  • Chemistry, Medicine
  • Biochimica et biophysica acta
  • 23 March 1971
Abstract Isoelectric points of α-chymotrypsin (EC 3.4.4.5) and its inactive derivative produced by reaction with l -1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK) were studied by isoelectricExpand
  • 48
  • 1
Rapid estimation of the molecular weights of protein polypeptide chains using high-pressure liquid chromatography in 6 M guanidine hydrochloride.
  • N. Ui
  • Chemistry, Medicine
  • Analytical biochemistry
  • 1 August 1979
Abstract An attempt was made to perform high-speed gel filtration in 6 m guanidine hydrochloride. A column of silica-based aqueous gels, G 3000 SW or G 4000 SW, in conjunction with a high-pressureExpand
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