Generation of hyaline cartilaginous tissue from mouse adult dermal fibroblast culture by defined factors.
- K. Hiramatsu, S. Sasagawa, Hidetatsu Outani, K. Nakagawa, H. Yoshikawa, N. Tsumaki
- Biology, MedicineJournal of Clinical Investigation
- 1 February 2011
The doxycycline-inducible induction system demonstrated that induced cells are able to respond to chondrogenic medium by expressing endogenous Sox9 and maintain chondrogensic potential after substantial reduction of transgene expression, which could lead to the preparation of hyaline cartilage directly from skin, without generating iPS cells.
Generation of Scaffoldless Hyaline Cartilaginous Tissue from Human iPSCs
- A. Yamashita, M. Morioka, N. Tsumaki
- BiologyStem Cell Reports
- 26 February 2015
Bone Morphogenetic Proteins in Bone Stimulate Osteoclasts and Osteoblasts During Bone Development
- M. Okamoto, J. Murai, H. Yoshikawa, N. Tsumaki
- Biology, MedicineJournal of Bone and Mineral Research
- 1 July 2006
In this study, overexpression of noggin, a BMP antagonist, in developing bone caused significantly decreased osteoclast number as well as bone formation rate, resulting in increased bone mass with…
SIK3 is essential for chondrocyte hypertrophy during skeletal development in mice
- S. Sasagawa, H. Takemori, N. Tsumaki
- Medicine, BiologyDevelopment
- 15 March 2012
An essential role for SIK3 is demonstrated in facilitating chondrocyte hypertrophy during skeletogenesis and growth plate maintenance and the Sik3-deficient cartilage phenotype was rescued by transgenic SIK 3 expression in the humerus.
Statin treatment rescues FGFR3 skeletal dysplasia phenotypes
- A. Yamashita, M. Morioka, N. Tsumaki
- Medicine, BiologyNature
- 25 September 2014
It is found that statin treatment can rescue patient-specific induced pluripotent stem cell (iPSC) models and a mouse model of FGFR3 skeletal dysplasia and suggest that statins could represent a medical treatment for infants and children with TD1 and ACH.
Osterix Regulates Calcification and Degradation of Chondrogenic Matrices through Matrix Metalloproteinase 13 (MMP13) Expression in Association with Transcription Factor Runx2 during Endochondral…
- R. Nishimura, M. Wakabayashi, T. Yoneda
- Biology, MedicineJournal of Biological Chemistry
- 6 August 2012
Osterix is an essential transcription factor for endochondral ossification that functions downstream of Runx2 and induces MMP13 during chondrocyte differentiation and microarray analysis revealed that matrix metallopeptidase 13 (MMP13) is an important target of Osterix.
Wwp2 is essential for palatogenesis mediated by the interaction between Sox9 and mediator subunit 25.
- Yukio Nakamura, Koji Yamamoto, H. Akiyama
- Biology, MedicineNature Communications
- 22 March 2011
Evidence is provided that the regulatory interaction between Sox9, Wwp2 and Med25 defines the Sox9 transcriptional mechanisms of chondrogenesis in the forming palate.
Sox9 sustains chondrocyte survival and hypertrophy in part through Pik3ca-Akt pathways
- D. Ikegami, H. Akiyama, N. Tsumaki
- Biology, MedicineDevelopment
- 15 April 2011
Endochondral bone formation-related Sox9 expression starts in mesenchymal progenitors, continues in the round and flat chondrocyte stages at high levels, and ceases just prior to the hypertrophic chondROcyte stage, suggesting that continued expression of Sox9just prior to hypertrophy is necessary for chondrosarcomaHypertrophy induced by Sox9.
Role of CDMP-1 in Skeletal Morphogenesis: Promotion of Mesenchymal Cell Recruitment and Chondrocyte Differentiation
- N. Tsumaki, Kazuhiro Tanaka, Yoshihiko Yamada
- Biology, MedicineJournal of Cell Biology
- 11 January 1999
Results indicate that CDMP-1 antagonizes the ventralization signals from the notochord, and suggests a molecular mechanism by which CD MP-1 regulates the formation, growth, and differentiation of the skeletal elements.
Bone Morphogenetic Protein Signals Are Required for Cartilage Formation and Differently Regulate Joint Development During Skeletogenesis
- N. Tsumaki, T. Nakase, H. Yoshikawa
- Biology, EngineeringJournal of Bone and Mineral Research
- 1 May 2002
Together with the common effect on the cartilage overproduction by Bmp4 and GDF5 overactivation, loss of cartilage by inactivation of multiple BMPs in Noggin transgenic mice indicates that signals for cartilage production are reinforced by multiple B MPs exclusively.
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