• Publications
  • Influence
Caspases: enemies within.
This work has shown that understanding caspase regulation is intimately linked to the ability to rationally manipulate apoptosis for therapeutic gain. Expand
Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis
A potent peptide aldehyde inhibitor has been developed and shown to prevent apoptotic events in vitro, suggesting that apopain/CPP32 is important for the initiation of apoptotic cell death. Expand
A Combinatorial Approach Defines Specificities of Members of the Caspase Family and Granzyme B
A novel method employing a positional scanning substrate combinatorial library to rigorously define individual specificities of caspase and granzyme B proteases divides these proteases into three distinct groups and suggests that several have redundant functions. Expand
A novel heterodimeric cysteine protease is required for interleukin-1βprocessing in monocytes
Purification and cloning of the complementary DNA indicates that IL-lβ-converting enzyme is composed of two nonidentical subunits that are derived from a single proenzyme, possibly by autoproteolysis. Expand
Caspases: killer proteases.
Caspases (cysteinyl aspartate-specific proteinases) mediate highly specific proteolytic cleavage events in dying cells, which collectively manifest the apoptotic phenotype. The key and central roleExpand
Inhibition of Human Caspases by Peptide-based and Macromolecular Inhibitors*
The results obtained with peptide-based inhibitors are in accord with those predicted from the substrate specificity studies described earlier, and the cowpox serpin CrmA is a potent and selective inhibitor of Group I and most Group III caspases, suggesting that this virus facilitates infection through inhibition of both apoptosis and the host inflammatory response. Expand
Human ICE/CED-3 Protease Nomenclature
A committee of several scientists who have been involved in the identification and characterization of these enzymes have formed a committee, with the objective of proposing a nomenclature for the human members of this protease family that is sensible and easy to use. Expand
The target of ezetimibe is Niemann-Pick C1-Like 1 (NPC1L1).
A binding assay is developed and shown that labeled ezetimibe glucuronide binds specifically to a single site in brush border membranes and to human embryonic kidney 293 cells expressing NPC1L1, which unequivocally establish NPC1 L1 as the direct target of ezETimibe and should facilitate efforts to identify the molecular mechanism of cholesterol transport. Expand
Involvement of Caspases in Proteolytic Cleavage of Alzheimer’s Amyloid-β Precursor Protein and Amyloidogenic Aβ Peptide Formation
Caspases appear to play a dual role in proteolytic processing of APP and the resulting propensity for Aβ peptide formation, as well as in the ultimate apoptotic death of neurons in Alzheimer's disease. Expand
Cleavage of huntingtin by apopain, a proapoptotic cysteine protease, is modulated by the polyglutamine tract
The results show that huntingtin is cleaved by cysteine proteases and suggest that HD might be a disorder of inappropriate apoptosis, providing an explanation for the gain–of–function associated with GAG expansion. Expand