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Challenges and prospects for the establishment of embryonic stem cell lines of domesticated ungulates.
TLDR
The identification of appropriate stem cell markers, functional cytokine pathways, and key pluripotency-maintaining factors along with the release of more comprehensive bovine and porcine genomes, provide encouragement for establishment of ungulate ES cell lines in the near future. Expand
Genetically enhanced cows resist intramammary Staphylococcus aureus infection
TLDR
The results indicate that genetic engineering can provide a viable tool for enhancing resistance to disease and improve the well-being of livestock with as little as 3 mg/ml of lysostaphin in milk. Expand
Cell Donor Influences Success of Producing Cattle by Somatic Cell Nuclear Transfer
TLDR
It is concluded that a significant component in determining somatic cell nuclear transfer success is the source of the nuclear donor cells. Expand
Alkaline phosphatase staining of pig and sheep epiblast cells in culture
TLDR
Pig and sheep epiblast cells tested for the presence of alkaline phosphatase (AP), a biochemical marker characteristic of mouse embryonic stem cells, were positive for AP staining both at isolation from the blastocyst and after primary in vitro culture. Expand
Expression analysis of pluripotency factors in the undifferentiated porcine inner cell mass and epiblast during in vitro culture
TLDR
The data demonstrate the potential use of some pluripotency factors as markers of porcine epiblast stem cells and indicate that the in vitro environment may influence the cultured epiblasts's developmental state. Expand
The Pursuit of ES Cell Lines of Domesticated Ungulates
TLDR
Thorough molecular characterizations, including the elucidation of stem cell “marker” signaling cascade hierarchy, species-appropriate pluripotency markers, and pluripOTency-associated chromatin alterations in the genomes of ungulate species, should improve the chances of developing efficient, reproducible technologies for the establishment of ES cell lines of economically important species. Expand
Bovine Blastocyst-Derived Trophectoderm and Endoderm Cell Cultures: Interferon Tau and Transferrin Expression as Respective In Vitro Markers
TLDR
This simple coculture method for the in vitro propagation of bovine trophectoderm and endoderm provides a system for assessing their biology in vitro. Expand
Culturing the epiblast cells of the pig blastocyst
TLDR
Results demonstrate the in vitro pluripotency of pig epiblast cells and indicate the epiblasts can be a source for deriving various specialized cell cultures or cell lines. Expand
Fibroblast growth factor-2 is expressed by the bovine uterus and stimulates interferon-tau production in bovine trophectoderm.
TLDR
FGF-2 is present in the uterine lumen during early pregnancy and increases IFNT mRNA and protein abundance in trophectoderm, which suggests that this uterine-derived factor plays an active role in regulating the establishment and maintenance of pregnancy in ruminants. Expand
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