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Cloning and nucleotide sequence of fosfomycin biosynthetic genes of Streptomyces wedmorensis
The biosynthetic pathway for production of the antibiotic fosfomycin by Streptomyces wedmorensis consists of four steps including the formation of a C-P bond and an epoxide. Fosfomycin productionExpand
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Enzymological Characteristics of Avian Influenza A Virus Neuraminidase
Neuraminidases of 18 strains of avian influenza A virus were examined by both colorimetric and fluorometric assays using fetuin and 4‐methylumbelliferyl‐N‐Ac‐α‐d‐neuraminide as substrates,Expand
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Enhancing effect of centrifugation on isolation of influenza virus from clinical specimens.
The use of centrifugation (700 x g, 60 min) in a plaque assay markedly increased (mean, 2.9-fold) the infectivity of all 42 influenza virus strains tested, compared with no centrifugation. Of 13Expand
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Fluorometric measurement of neuraminidase activity of influenza viruses.
A rapid and simple assay method for determining neuraminidase activity was investigated fluorometrically with 4-methylumbelliferyl-N-Ac-a-D-neuraminide as a substrate. The time required forExpand
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Enzymological heterogeneity of influenza B virus neuraminidase demonstrated by the fluorometric assay method.
The neuraminidase activity of 26 strains of influenza B virus isolated from all over the world was investigated colorimetrically, using fetuin as a substrate, and fluorometrically, usingExpand
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Lysosomal enzyme release from guinea pig polymorphonuclear leukocytes by influenza virus.
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