RECOGNIZING DINOFLAGELLATE SPECIES USING ITS rDNA SEQUENCES 1
The results indicate that a between‐species uncorrected genetic distance of p could be used to delineate most free‐living dinoflagellate species, however, recently evolved species, may have ITS p values <0.04 and would require more extensive morphological and genetic analyses to resolve.
Herpes virus in juvenile Pacific oysters Crassostrea gigas from Tomales Bay, California, coincides with summer mortality episodes.
- C. Friedman, Robyn M Estes, K. Reece
- Environmental Science, BiologyDiseases of Aquatic Organisms
- 25 January 2005
Hemocytosis, diapedesis and hemocyte degeneration characterized by nuclear pycnosis and fragmentation were observed in infected oysters, which is consistent with previous observations of OsHV infections in France.
IDENTIFICATION OF PFIESTERIA PISCICIDA (DINOPHYCEAE) AND PFIESTERIA‐LIKE ORGANISMS USING INTERNAL TRANSCRIBED SPACER‐SPECIFIC PCR ASSAYS 1
These PCR assays had a minimum sensitivity of 100 cells in a 100‐mL sample and were successfully used to detect PLOs in the St. Johns River system in Florida, USA.
Increased Virulence in an Introduced Pathogen: Haplosporidium nelsoni (MSX) in the Eastern Oyster Crassostrea virginica.
Results document greatly increased virulence in a naive host-parasite association and reinforce potential dangers of intentional, but improper, introductions of exotic marine organisms for aquaculture or resource restoration.
Bonamia perspora n. sp. (Haplosporidia), a Parasite of the Oyster Ostreola equestris, is the First Bonamia Species Known to Produce Spores
Histopathology, electron microscopy, and molecular phylogenetic analysis support the designation of a new parasite species, Bonamia perspora n.
Haplosporidiosis in the Pacific oyster Crassostrea gigas from the French Atlantic coast.
In situ hybridization of infected tissue sections was conducted with DNA probes for oyster-infecting haplosporidians, and a series of primers was designed to amplify short regions of small subunit ribosomal DNA (SSU rDNA) from most haplosporaidians.
MOLECULAR PHYLOGENY OF THE HAPLOSPORIDIA BASED ON TWO INDEPENDENT GENE SEQUENCES
In almost all analyses, the Haplosporidia was sister to the Cercozoa with moderate bootstrap and jackknife support, and analysis with actin amino acid sequences alone grouped haplosporidians with the foraminiferans and cercozoans.
Molecular diagnostics, field validation, and phylogenetic analysis of Quahog Parasite Unknown (QPX), a pathogen of the hard clam Mercenaria mercenaria.
- N. Stokes, L. M. Ragone Calvo, K. Reece, E. Burreson
- BiologyDiseases of Aquatic Organisms
- 10 December 2002
Phylogenetic analyses based on theQPX and Labyrinthulomycota sequences confirmed earlier reports that QPX is a member of this phylum, but could not definitively demonstrate that all of the QPx organisms were the same species.
Phylogenetics of Bonamia parasites based on small subunit and internal transcribed spacer region ribosomal DNA sequence data.
Phylogenetic analyses using parsimony and Bayesian methods revealed one species, B. exitiosa, to be widely distributed, infecting 7 oyster species from Australia, New Zealand, Argentina, eastern and western USA, and Tunisia, and more limited host and geographic distributions of B. ostreae and B. roughleyi were revealed.
Natural and cultured populations of the mangrove oyster Saccostrea palmula from Sinaloa, Mexico, infected by Perkinsus marinus.