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Site-directed modification of DNA duplexes by chemical ligation.
The efficiency of chemical ligation method have been demonstrated by assembling a number of DNA duplexes with modified sugar phosphate backbone by performing condensation on a tetradecanucleotide template of hexa(penta)- and undecan nucleoside residue using water-soluble carbodiimide as a condensing agent. Expand
A new method for the synthesis of oligodeoxyribonucleotides containing internucleotide phosphoramidate bonds
Abstract Condensation of 5′-0- and N-blocked deoxynucleoside 3′-0-methyl-H-phosphonates (I) with 5′-amino-2′,5′-dideoxynucleosides (II) in the presence of CCl 4 and Et 3 N produces dinucleosideExpand
The use of BrCN for assembling modified DNA duplexes and DNA-RNA hybrids; comparison with water-soluble carbodiimide.
Using both condensing reagents, the condensation of oligonucleotides containing ribo-segments on the DNA template was studied and efficiency of the chemical ligation of RNA oligomers was shown to be much lower than that of DNA analogues. Expand
Chemical reactions within DNA duplexes Cyanogen bromide as an effective oligodeoxyribonucleotide coupling agent
Cyanogen bromide was found to condense oligodeoxyribonucleotides on a complementary template in aqueous solution and CNBr proved to be useful for incorporation of phosphoramidate or pyrophosphate internucleotide bonds in DNA duplexes. Expand
An active-site-directed adenosine triphosphate analogue binds to the beta-subunits of factor F1 mitochondrial adenosine triphosphatase with its triphosphate moiety.
The results suggest that ATP may also bind to the beta-subunit of the adenosine triph phosphatase with its triphosphate moiety. Expand
Investigation of activation of phosphate groups in mono- and oligonucleotides with mesitoyl chloride
It has been demonstrated with the use of 31P NMR pulsed spectroscopy that the reaction of mesitoyl chloride (MsCOCl) both with terminal and internucleotide phosphate groups pA, d(MeOTr)TpT and dpTpTExpand
Modification of end phosphate gruops in mono- and oligonucleotides.
Mixed anhydrides studied may be efficiently used for affinity labeling of proteins and nucleic acids and also as material for preparating reagents for template reactions. Expand
Chemical ligation of DNA: the first non-enzymatic assembly of a biologically active gene.
Along with the fully chemical assembly of synthetic genes, a rapid and sufficiently reliable method for determining their ribosome-binding properties was developed and it is found that the polymerase inhibition technique (toe-printing) is a good way to ascertain that translation initiation complexes form in the case of single-stranded DNAs. Expand