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Structural insight into the ligand specificity of a thermostable family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum.
TLDR
The paucity of direct hydrogen bonds with the aglycone moiety and the flexible conformation adopted by Trp(178), which stacks against the sugar at the +1 subsite, provide a structural explanation for the plasticity in substrate specificity displayed by the clostridial arabinofuranosidase. Expand
The Location of the Ligand-binding Site of Carbohydrate-binding Modules That Have Evolved from a Common Sequence Is Not Conserved*
TLDR
Structural data define a superfamily of CBMs, comprising CBM4, CBM6, and CBM22, and demonstrate that, although CBMs have evolved from a relatively small number of ancestors, the structural elements involved in ligand recognition have been assembled at different locations on the ancestral scaffold. Expand
Mannose Foraging by Bacteroides thetaiotaomicron
TLDR
The identification of GH-A clan and GH2 specific residues in the active site of BtMan2A explains why this enzyme is able to harness substrate binding at the proximal glycone binding site more efficiently than mannan-hydrolyzing glycoside hydrolases in related enzyme families. Expand
Mechanistic insights into a Ca2+-dependent family of alpha-mannosidases in a human gut symbiont.
TLDR
It is shown that GH92 mannosidases are alpha-mannosidases that act via a single displacement mechanism to utilize host N-glycans to exploit dietary polysaccharides and host glycans as nutrients. Expand
Evidence for a dual binding mode of dockerin modules to cohesins
TLDR
The dual binding mode is predicted to impart significant plasticity into the orientation of the catalytic subunits within this supramolecular assembly, which reflects the challenges presented by the degradation of a heterogeneous, recalcitrant, insoluble substrate by a tethered macromolecular complex. Expand
Structural and biochemical evidence for a boat-like transition state in beta-mannosidases.
TLDR
Support for a B2,5 transition state during enzymatic beta-mannosidase hydrolysis should also facilitate the design and exploitation of transition state mimics for the inhibition of retaining alpha-mannOSidases--an area that is emerging for anticancer therapeutics. Expand
Understanding how diverse beta-mannanases recognize heterogeneous substrates.
TLDR
The biochemical properties and crystal structures of both a GH5 and a GH26 mannanase are reported and the contributions to substrate specificity in these enzymes are described and the biological rationale for the variable recognition of Man- and Glc-configured sugars by beta-mannanases is discussed. Expand
Structure of a group A streptococcal phage-encoded virulence factor reveals a catalytically active triple-stranded beta-helix.
TLDR
It is demonstrated that HylP1, a phage tail-fiber protein responsible for the digestion of the S. pyogenes hyaluronan capsule during phage infection, is ahyaluronate lyase, and 3D structure reveals an unusual triple-stranded beta-helical structure and provides insight into the structural basis forphage tail assembly and the role of phage Tail proteins in virulence. Expand
Structures of two truncated phage-tail hyaluronate lyases from Streptococcus pyogenes serotype M1.
The crystal structures of truncated forms of the Streptococcus pyogenes phage-encoded hyaluronate lyases HylP2 and HylP3 were determined by molecular replacement to 1.6 and 1.9 A resolution,Expand
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