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A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes.
A nomenclature is described for restriction endonucleases, DNA methyltransferases, homing endonucleases and related genes and gene products. It provides explicit categories for the many differentExpand
Nanometal surface energy transfer in optical rulers, breaking the FRET barrier.
This report reports the first successful application of a dipole-surface type energy transfer from a molecular dipole to a nanometal surface that more than doubles the traditional Forster range (220 A) and follows a 1/R(4) distance dependence. Expand
The Inherent Processivity of the Human de Novo Methyltransferase 3A (DNMT3A) Is Enhanced by DNMT3L*
It is shown that DNMT3A methylates 5–8 CpG sites on human promoters before 50% of the initially bound enzyme dissociates from the DNA, providing a mechanism that ensures that multiple C pG sites undergo methylation for transcriptional regulation and silencing of newly integrated viral DNA. Expand
Mutations in DNA Methyltransferase (DNMT3A) Observed in Acute Myeloid Leukemia Patients Disrupt Processive Methylation
The results provide a possible mechanism that accounts for how DNMT3A mutations may contribute to oncogenesis and its progression, and provide a plausible explanation for changes in epigenetic regulation in AML patients. Expand
Surface-enhanced Raman spectroscopy for DNA detection by nanoparticle assembly onto smooth metal films.
AFM analysis indicates that strong SERS signal intensity arises from only a few surface-bound nanoparticles which generate an enhancement factor ∼105−106 greater than the metal film alone. Expand
Fabrication of Ag@SiO(2)@Y(2)O(3):Er nanostructures for bioimaging: tuning of the upconversion fluorescence with silver nanoparticles.
We demonstrated that the nanostructures comprising silver cores and dense layers of Y(2)O(3):Er separated by a silica shell are an excellent model system to study the interaction between upconversionExpand
A heterogeneous PNA-based SERS method for DNA detection.
A simple method for ssDNA detection based on SERS signals and PNA slides is reported, revealing that the distribution of nanoparticles is random and that approximately 1 in 10 aggregate sites is responsible for the SERS spectra. Expand
Oligomerization of DNMT3A Controls the Mechanism of de Novo DNA Methylation
The results provide a structural mechanism for the regulation of DNMT3A activity and epigenetic imprinting and Tetramerization with the known interacting partnerDNMT3L rescues processive catalysis, demonstrating that protein binding at the DN MT3A tetramer interface can modulate methylation patterning. Expand
The early expressed HIV-1 genes regulate DNMT1 expression
HIV-1 mediated induction of DNMT1 results from over-expression of the early expressed HIV-1 proteins, and this induction is prevented with resveratrol which interferes with the transcription factor AP1 pathway. Expand
Identification of a second DNA binding site in human DNA methyltransferase 3A by substrate inhibition and domain deletion.
It is shown that the conserved PWWP domain is necessary for substrate inhibition and forms at least part of the allosteric DNA binding site, demonstrating that DNMT3A has a second binding site for DNA. Expand