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Expression systems for heterologous production of antimicrobial peptides

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Genetic basis for hyper production of hyaluronic acid in natural and engineered microorganisms

For the first time this review has compiled the molecular and genetic bases for natural HA production in microorganisms together with the main strategies employed for heterologous production of HA.
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Lovastatin production: From molecular basis to industrial process optimization.

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Comparison of Yeasts as Hosts for Recombinant Protein Production

A comparative analysis is done listing the advantages and disadvantages of using each host regarding the availability of genetic tools, strategies for cultivation in bioreactors, and the main techniques utilized for protein purification.
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Kinetic modelling reveals current limitations in the production of ethanol from xylose by recombinant Saccharomyces cerevisiae.

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A functional screen for recovery of 4'-phosphopantetheinyl transferase and associated natural product biosynthesis genes from metagenome libraries.

Nearly half of the PPTases recovered in the screening of two small-insert soil metagenome libraries were genetically linked to recognizable secondary metabolite biosynthetic genes, demonstrating that PPTase-targeting functional screens can be used for efficient recovery of natural product gene clusters from metagenomes libraries.
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Isolation of xylose isomerases by sequence- and function-based screening from a soil metagenomic library

For the first time, the screening of a soil metagenomic library in E. coli resulted in the successful isolation of two active XIs, suggesting that future screening for XI activity from soil should be pursued directly using yeast as a host.
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Identification of common traits in improved xylose‐growing Saccharomyces cerevisiae for inverse metabolic engineering

Four recombinant Saccharomyces cerevisiae strains with enhanced xylose growth were compared with two control strains through genome‐wide transcription analysis in order to identify novel targets for inverse metabolic engineering.
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Xylanases from Cryptococcus flavus isolate I-11: Enzymatic profile, isolation and heterologous expression of CfXYN1 in Saccharomyces cerevisiae

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New edge of antibiotic development: antimicrobial peptides and corresponding resistance

Although there are many studies occurring at bench scale, indicating that genetic modified plants over producing defensins should appear in the market within the next years, it is important to emphasize that antimicrobial peptides have other potential applications since some of them have also antitumoral and imunomodulator activities.
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