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Kinetic modelling reveals current limitations in the production of ethanol from xylose by recombinant Saccharomyces cerevisiae.
Saccharomyces cerevisiae lacks the ability to ferment the pentose sugar xylose that is the second most abundant sugar in nature. Therefore two different xylose catabolic pathways have beenExpand
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Isolation of xylose isomerases by sequence- and function-based screening from a soil metagenomic library
BackgroundXylose isomerase (XI) catalyses the isomerisation of xylose to xylulose in bacteria and some fungi. Currently, only a limited number of XI genes have been functionally expressed inExpand
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Identification of common traits in improved xylose‐growing Saccharomyces cerevisiae for inverse metabolic engineering
Four recombinant Saccharomyces cerevisiae strains with enhanced xylose growth (TMB3400, C1, C5 and BH42) were compared with two control strains (TMB3399, TMB3001) through genome‐wide transcriptionExpand
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Identification and characterization of putative xylose and cellobiose transporters in Aspergillus nidulans
BackgroundThe conversion of lignocellulosic biomass to biofuels (second-generation biofuel production) is an environmentally friendlier alternative to petroleum-based energy sources. EnzymaticExpand
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Rhamnolipids production from sucrose by engineered Saccharomyces cerevisiae
Biosurfactants are biological tensioactive agents that can be used in the cosmetic and food industries. Rhamnolipids are glycolipid biosurfactants naturally produced by Pseudomonas aeruginosa and areExpand
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Novel homologous lactate transporter improves l-lactic acid production from glycerol in recombinant strains of Pichia pastoris
BackgroundCrude glycerol is the main byproduct of the biodiesel industry. Although it can have different applications, its purification is costly. Therefore, in this study a biotechnological routeExpand
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Xylanases from Cryptococcus flavus isolate I-11: Enzymatic profile, isolation and heterologous expression of CfXYN1 in Saccharomyces cerevisiae
The aim of this study was to characterize the xylanolytic activity of Cryptococcus flavus isolate I-11. This microorganism was isolated from the Brazilian Cerrado, and enzyme plate assays showed thatExpand
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Comparative assessment of fermentative capacity of different xylose-consuming yeasts
BackgroundUnderstanding the effects of oxygen levels on yeast xylose metabolism would benefit ethanol production. In this work, xylose fermentative capacity of Scheffersomyces stipitis, SpathasporaExpand
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Flotation as a tool for indirect DNA extraction from soil
Nowadays, soil diversity is accessed at molecular level by the total DNA extraction of a given habitat. However, high DNA yields and purity are difficult to achieve due to the co-extraction ofExpand
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The deletion of YLR042c improves ethanolic xylose fermentation by recombinant Saccharomyces cerevisiae
In a recent study combining transcriptome analyses of a number of recombinant laboratory and industrial S. cerevisiae strains with improved xylose utilization and their respective control strains,Expand
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