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Molecular-phylogenetic characterization of microbial community imbalances in human inflammatory bowel diseases
Patient stratification by GI microbiota provides further evidence that CD represents a spectrum of disease states and suggests that treatment of some forms of IBD may be facilitated by redress of the detected microbiological imbalances. Expand
Impact of Culture-Independent Studies on the Emerging Phylogenetic View of Bacterial Diversity
Our perspective on microbial diversity has improved enormously over the past few decades. In large part this has been due to molecular phylogenetic studies that objectively relate organisms.Expand
Novel Division Level Bacterial Diversity in a Yellowstone Hot Spring
ABSTRACT A culture-independent molecular phylogenetic survey was carried out for the bacterial community in Obsidian Pool (OP), a Yellowstone National Park hot spring previously shown to containExpand
A molecular view of microbial diversity and the biosphere.
  • N. Pace
  • Biology, Medicine
  • Science
  • 2 May 1997
Over three decades of molecular-phylogenetic studies, researchers have compiled an increasingly robust map of evolutionary diversification showing that the main diversity of life is microbial,Expand
Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses.
A protocol is described for rapidly generating large blocks of 16S rRNA sequence data without isolation of the 16 S rRNA or cloning of its gene, and its phylogenetic usefulness is evaluated by examination of several 17S rRNAs whose gene sequences are known. Expand
Perspectives on archaeal diversity, thermophily and monophyly from environmental rRNA sequences.
Phylogenetic analysis of ribosomal RNA sequences obtained from uncultivated organisms of a hot spring in Yellowstone National Park reveals several novel groups of Archaea, many of which diverged fromExpand
Remarkable archaeal diversity detected in a Yellowstone National Park hot spring environment.
The large number of distinct archaeal sequence types retrieved from this single hot spring was unexpected and demonstrates that Crenarchaeota is a much more diverse group than was previously suspected. Expand
Microbial Diversity in a Hydrocarbon- and Chlorinated-Solvent-Contaminated Aquifer Undergoing Intrinsic Bioremediation
It is hypothesized that the terminal step of hydrocarbon degradation in the methanogenic zone of the aquifer is aceticlastic methanogenesis and that the microorganisms represented by these two sequence types occur in syntrophic association. Expand
Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells.
Quantitative microfluorimetry shows that the amount of an rRNA-specific probe that binds to Escherichia coli varies with the ribosome content and therefore reflects growth rate. Expand
Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells
Examination of collections of 16S rRNA sequences revealed sequence domains that were unique to (and invariant within) the three primary lines of cellular descent: the archaebacteria, the eubacteria,Expand