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Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice
TLDR
Surprisingly, the number of focal adhesions was increased in FAK-deficient cells, suggesting that FAK may be involved in the turnover of focalAdhesion contacts during cell migration.
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Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells
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Characterization of human embryonic stem cell lines by the International Stem Cell Initiative
The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and…
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Nanog expression in mouse germ cell development.
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Efficient gene transfer into the embryonic mouse brain using in vivo electroporation.
TLDR
A novel method to introduce DNA into in/exo utero embryonic mouse brains at various stages by using electroporation, which will be a powerful tool to characterize gene function in various settings due to its high efficiency and localized gene expression.
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The TDRD9-MIWI2 complex is essential for piRNA-mediated retrotransposon silencing in the mouse male germline.
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Efficient establishment of human embryonic stem cell lines and long-term maintenance with stable karyotype by enzymatic bulk passage.
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Octamer and Sox Elements Are Required for Transcriptional cis Regulation of Nanog Gene Expression
TLDR
Nanog transcription may be regulated through an interaction between Oct4 and Sox2 or a novel pluripotential cell-specific Sox element-binding factor which is prominent in ES cells.
View on ASMUSA
Autonomous transition into meiosis of mouse fetal germ cells in vitro and its inhibition by gp130-mediated signaling.
TLDR
A novel activity of gp130-mediated signaling, known to promote survival/growth of PGCs and also to inhibit the differentiation of embryonic stem cells, suppressed the expression of Scp3 in P GCs and inhibited the following formation of axial cores in vitro, supporting the hypothesis that PGC's are capable of entering meiosis before arriving at the UGR.
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Defining early lineage specification of human embryonic stem cells by the orchestrated balance of canonical Wnt/β-catenin, Activin/Nodal and BMP signaling
TLDR
It is shown that stabilized expression of β-catenin perturbed human embryonic stem (hES)-cell self-renewal, such that up to 80% of the hES cells developed into the primitive streak (PS)/mesoderm progenitors, reminiscent of early mammalian embryogenesis.
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