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Purification and properties of (S)-tetrahydroprotoberberine oxidase from suspension-cultured cells of Berberis wilsoniae.
This catalyst was responsible for the conversion of (S)-tetrahydrocolumbamine to the key intermediate, columbamine, in the metabolic pathway leading to berberine, jatrorrhizine and palmatine. Expand
(S)‐norlaudanosoline synthase: The first enzyme in the benzylisoquinoline biosynthetic pathway
Isoquinoline alkaloids form the largest group of alkaloids in the plant kingdom. Numerous publications deal with aspects of the biosynthesis of these compounds in vivo [ 11, while isoquinolineExpand
Phenolic and iridoid glycosides from Strychnos axillaris.
Five phenolic glycosides 1-5 and an iridoid glucoside 6 were isolated from the dried barks and woods of Strychnosaxillaris and their structures were determined by application of spectroscopic and chemical methodologies. Expand
Indole alkaloids and other constituents of Rauwolfia serpentina.
From the dried roots of Rauwolfia serpentina were isolated five new indole alkaloids, a new iridoid glucoside, 7-epiloganin, and a new sucrose derivative, 6'-O-(3,4,5-trimethoxybenzoyl)glomeratose A (7), together with 20 known compounds. Expand
Benzylisoquinoline Biosynthesis by Cultivated Plant Cells and Isolated Enzymes
It could be demonstrated that the transport of these compounds into vacuoles of Fumaria capreolata does not proceed by an ion-trap mechanism, but rather involves an active ATP-requiring, stereospecific carrier system acting only on the (S)and not on the @?)-enantiomer. Expand
The berberine bridge forming enzyme in tetrahydroprotoberberine biosynthesis
The enzyme responsible for the berberine bridge formation was purified to homogeneity and shown to catalyze, in the presence of oxygen, the conversion of (S)-reticuline, (S)-protosinomenine andExpand
Partial Purification and Properties of S-Adenosylmethionine: (R), (S)-Norlaudanosoline-6-O-Methyltransferase from Argemone platyceras Cell Cultures.
A new enzyme, S-adenosylmethionine: (R), (S)-norlaudanosoline-6-O-methyltransferase, was isolated from the soluble protein extract of A. PLATYCERAS cell cultures and purified approximately 80-fold.Expand
Partial Purification and Properties of (S)-Norlaudanosoline Synthase from Eschscholtzia tenuifolia Cell Cultures.
By means of isoelectric focusing and disc-gel electrophoresis evidence was obtained for the existence of four norlaudanosoline synthase isoenzymes, none of which catalyses the reaction of dopamine with 3,4-dihydroxyphenylpyruvate, the key intermediate in the formation of isoquinoline alkaloids occurring in the plant kingdom. Expand