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LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing
It is demonstrated that the rat microtubule‐associated protein 1 light chain 3 (LC3), a homologue of Apg8p essential for autophagy in yeast, is associated to the autophagosome membranes after processing. Expand
Methods in Mammalian Autophagy Research
Methods to monitor autophagy and to modulate autophagic activity are discussed, with a primary focus on mammalian macroautophagy. Expand
Bcl-2 Antiapoptotic Proteins Inhibit Beclin 1-Dependent Autophagy
Bcl-2 not only functions as an antiapoptotic protein, but also as an antiautophagy protein via its inhibitory interaction with Beclin 1, which may help maintain autophagy at levels that are compatible with cell survival, rather than cell death. Expand
Autophagy fights disease through cellular self-digestion
Understanding autophagy may ultimately allow scientists and clinicians to harness this process for the purpose of improving human health, and to play a role in cell death. Expand
Autophagy: Renovation of Cells and Tissues
It is explored how recent mouse models in combination with advances in human genetics are providing key insights into how the impairment or activation of autophagy contributes to pathogenesis of diverse diseases, from neurodegenerative diseases such as Parkinson disease to inflammatory disorders such as Crohn disease. Expand
The role of Atg proteins in autophagosome formation.
The molecular mechanism of autophagosome formation is described with particular focus on the function of Atg proteins and the long-standing discussion regarding the origin of the autophagous membrane membrane. Expand
Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury
It is demonstrated that necroptosis contributes to delayed mouse ischemic brain injury in vivo through a mechanism distinct from that of apoptosis and offers a new therapeutic target for stroke with an extended window for neuroprotection. Expand
Autophagy: process and function.
  • N. Mizushima
  • Biology, Medicine
  • Genes & development
  • 15 November 2007
In this review, the process of autophagy is summarized, and the role of autophileagy is discussed in a process-based manner. Expand
In vivo analysis of autophagy in response to nutrient starvation using transgenic mice expressing a fluorescent autophagosome marker.
The results suggest that the regulation of autophagy is organ dependent and the role of Aut7/Apg8 is not restricted to the starvation response, and this transgenic mouse model is a useful tool to study mammalian autophagic regulation. Expand
How to Interpret LC3 Immunoblotting
This work has shown that it is important to measure the amount of LC3-II delivered to lysosomes by comparing LC2-II levels in the presence and absence ofLysosomal protease inhibitors, and that this amount can be compared between samples. Expand