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Analysis of intracellular and intraviral localization of the human cytomegalovirus UL53 protein.
The expression and localization of the UL53 gene product was analysed and showed that pUL53 is a new structural protein, suggesting a role compatible with the function hypothesized for UL31, its positional homologue in herpes simplex virus type 1. Expand
Monoclonal antibodies to human DNA topoisomerase I and the two isoforms of DNA topoisomerase II: 170- and 180-kDa isozymes.
Morphological studies performed by immunofluorescence indicate that the three groups of monoclonal antibodies (MoAbs) stain the nucleus with characteristic patterns, which can be compared with those obtained with polyclonal antibodies. Expand
Ataxin-3 is transported into the nucleus and associates with the nuclear matrix.
The results taken together with the finding of a nuclear localization signal in ataxin-3 indicate that the ataxIn-3 protein per se translocates to the nucleus and that an expanded glutamine repeat is not essential for this transport. Expand
Immunocytochemical detection of phosphatidylinositol 4,5-bisphosphate localization sites within the nucleus.
It is shown that a significant amount of the phospholipid is retained by the cytoskeleton and by the inner nuclear matrix in in situ matrix preparations and the sensitivity of the immunocytochemical reaction is capable of detecting quantitative variations of PIP2 nuclear content induced by agonists that modulate the signal transduction system at the nuclear level. Expand
Phosphatidylinositol 3‐kinase is recruited to a specific site in the activated IL‐1 receptor I
Investigation of the role of PI 3‐kinase in IL‐1 signalling indicates that it is likely to be an important mediator of someIL‐1 effects, providing docking sites for additional signalling molecules. Expand
Nuclear immunolocalization of P-glycoprotein in multidrug-resistant cell lines showing similar mechanisms of doxorubicin distribution.
In resistant cells, inhibition of P-glycoprotein activity was able to induce drug accumulation in the nuclei of resistant cells and to achieve cytotoxic effects comparable to those observed in sensitive cells, suggesting the possible involvement of nuclear P- glycoprotein in the regulation of subcellular doxorubicin distribution in multidrug-resistant cells. Expand
High-resolution detection of newly synthesized DNA by anti-bromodeoxyuridine antibodies identifies specific chromatin domains.
We analyzed the incorporation of bromodeoxyuridine (BrdUrd) into DNA in exponentially growing murine erythroleukemia cells (FLC-745), using fluorescent anti-BrdUrd antibodies with light microscopyExpand
Inhibition of phosphoinositide 3-kinase impairs pre-commitment cell cycle traverse and prevents differentiation in erythroleukaemia cells
PI 3-kinase is rate limiting for the completion of the first round cycle of cell division required for initiation of erythrocytic differentiation, and the late decrease of PI 3-Kinase associated with the differentiation process seems to be part of the programmed shut off of genes not needed in mature erythropoiesis. Expand
Phospholipid interactions in rat liver nuclear matrix.
From in vitro labelling studies, the released DNA is more recently synthesised than the bulk material, however the matrix bound RNA appears to label less rapidly than total nuclear RNA. Expand
Topology of inositol lipid signal transduction in the nucleus
It is demonstrated that nuclear inositol lipids exist in a nonmembranous form, linked to structural nuclear proteins of the inner nuclear matrix and suggested that it probably preceded in evolution the systems that are present at the cytoskeletal and cell membrane level. Expand