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Cdc13p: A Single-Strand Telomeric DNA-Binding Protein with a Dual Role in Yeast Telomere Maintenance
It is shown that CDC13 has two discrete roles at the telomere, with the use of two classes of mutations that perturbs a function required in vivo for telomerase regulation but not in vitro for enzyme activity.
Loss of ATRX, Genome Instability, and an Altered DNA Damage Response Are Hallmarks of the Alternative Lengthening of Telomeres Pathway
It is shown that loss of ATRX protein and mutations in the ATRRX gene are hallmarks of ALT–immortalized cell lines, and these attributes will facilitate the diagnosis and treatment ofALT positive human cancers.
The structure and function of telomerase reverse transcriptase.
The structure and integrity of telomeres are essential for genome stability and regulation by RNA-DNA, TERT- DNA, Tert-RNA, TTERT interactions, and TERN-associated proteins; and the relationship between telomerase enzymology and telomere maintenance is discussed.
Identification of Functionally Important Domains in the N-Terminal Region of Telomerase Reverse Transcriptase
Deletion analysis indicates that even the most N-terminal nonconserved region of yeast TERT (N region) is required for telomerase function, and it is proposed that all TERTs may have a bipartite organization, with an N-GQ domain connected to the other motifs through a flexible linker.
Stn1-Ten1 is an Rpa2-Rpa3-like complex at telomeres.
Structural and functional studies illustrate that, instead of being confined to budding yeast telomeres, the CST complex may represent an evolutionarily conserved RPA-like telomeric complex at the 3' overhangs that works in parallel with or instead of the well-characterized POT1-TPP1/TEBPalpha-beta complex.
A yeast protein that influences the chromatin structure of UASG and functions as a powerful auxiliary gene activator.
- D. Chasman, N. Lue, A. Buchman, J. LaPointe, Y. Lorch, R. Kornberg
- BiologyGenes & development
- 1 April 1990
Purified GRF2 binds to sequences found in many other UASs, in the 35S rRNA enhancer, at centromeres, and at telomeres and combines with a neighboring weak activator to give as much as a 170-fold enhancement.
Connections between transcriptional activators, silencers, and telomeres as revealed by functional analysis of a yeast DNA-binding protein
The stimulatory effect of GRFI binding sites at UASs for the PYK1 and ENO1 genes is significantly enhanced by flanking DNA elements, which converts this region to a transcriptional silencer.
SLX4 Assembles a Telomere Maintenance Toolkit by Bridging Multiple Endonucleases with Telomeres
Statistical positioning of nucleosomes by specific protein-binding to an upstream activating sequence in yeast.