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Ageing and vision: structure, stability and function of lens crystallins.
TLDR
The structure, assembly, interactions, stability and post-translational modifications of the crystallins are reviewed, not only in isolation but also as part of a multi-component system. Expand
A DNAJB chaperone subfamily with HDAC-dependent activities suppresses toxic protein aggregation.
TLDR
Combined data provide a functional link between HDACs and DNAJs in suppressing cytotoxic protein aggregation and DNAJB8 is (de)acetylated at two conserved C-terminal lysines that are not involved in substrate binding, but do play a role in suppressing protein aggregation. Expand
The gamma-crystallins and human cataracts: a puzzle made clearer.
TLDR
Findings are the first evidence of an involvement of CRYGC and support the role ofCRYGD in human cataract formation and help clarify the mechanisms of lens opacification. Expand
The γ-Crystallins and Human Cataracts: A Puzzle Made Clearer
TLDR
Findings are the first evidence of an involvement of CRYGC and support the role ofCRYGD in human cataract formation and help clarify the mechanisms of lens opacification. Expand
The X-ray Crystal Structure of Human γS-crystallin C-terminal Domain*
TLDR
The crystal structure of the C-terminal domain of human γS-crystallin has been solved at 2.4 Å resolution and shows that in the in vitro expressed protein, the buried cysteines remain reduced. Expand
Molecular basis of a progressive juvenile-onset hereditary cataract.
TLDR
Rec recombinant wild-type human gammaD crystallin (HGD) and its Arg-14 to Cys mutant (R14C) in Escherichia coli and show that R14C forms disulfide-linked oligomers, which markedly raise the phase separation temperature of the protein solution and strongly suggest that the observed cataract is triggered by the thiol-mediated aggregation of R 14C. Expand
Covalent dimerization of vascular permeability factor/vascular endothelial growth factor is essential for its biological activity. Evidence from Cys to Ser mutations.
TLDR
VPF mutants lacking cysteine residue 2 or 4 competed poorly for receptor binding of labeled VPF and had low biological activity, indicating that for efficient receptor binding and activation VPF needs to be a covalent dimer, unlike PDGF-BB. Expand
Induction and maintenance of differentiation of rat lens epithelium by FGF-2, insulin and IGF-1.
TLDR
Investigation was to determine whether insulin-like growth factor 1 (IGF-1) can also maintain differentiation and to include a cellular analysis of explants undergoing insulin-or IGF-maintained differentiation in vitro and showed that IGF-1, like insulin, can replace FGF-2 in directing the pulses of alpha-, beta- and gamma-crystallin gene expression once differentiation is initiated by FGF -2. Expand
Urochordate βγ-Crystallin and the Evolutionary Origin of the Vertebrate Eye Lens
TLDR
The conservation of the regulatory hierarchy controlling βγ-crystallin expression between organisms with and without a lens shows that the evolutionary origin of the lens was based on co-option of pre-existing regulatory circuits controlling the expression of a key structural gene in a primitive light-sensing system. Expand
Developmental expression of crystallin genes: in situ hybridization reveals a differential localization of specific mRNAs.
TLDR
The results show that gamma-crystallin RNA starts to accumulate as soon as visible elongation of epithelial cells occurs, during differentiation of the primary as well as the secondary fiber cells. Expand
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