Etheno-substituted nucleotides and coenzymes: fluorescence and biological activity.
- N. Leonard
- Biology, ChemistryCRC Critical Reviews in Biochemistry
The binding of e-substituted nucleotides or polynucleotides to proteins has been determined for F- and G-actin, heavy meromyosin, tobacco mosaic virus protein, gene 32 protein of bacteriophage T4, and chloroplast...
Synthetic spectroscopic models related to coenzymes and base pairs. V. Emission properties of NADH. Studies of fluorescence lifetimes and quantum efficiencies of NADH, AcPyADH, [reduced…
Synthesis of modified nucleoside 3',5'-bisphosphates and their incorporation into oligoribonucleotides with T4 RNA ligase.
- J. Barrio, M. C. Barrio, N. Leonard, T. England, O. Uhlenbeck
- Chemistry, BiologyBiochemistry
- 30 May 1978
A simple procedure is described to prepare nucleoside 3'(2'),5'-bisphosphates from the corresponding nucleosides with the use of pyrophosphoryl chloride. This method is rapid, gives nearly…
Fluorescent modification of adenosine-containing coenzymes. Biological activities and spectroscopic properties.
1,N2-ethenoguanine and N2,3-ethenoguanine. Synthesis and comparison of the electronic spectral properties of these linear and angular triheterocycles related to the Y bases.
Cytokinins: Structure/activity relationships☆
Synthetic spectroscopic models related to coenzymes and base pairs. II. Evidence for intramolecular base-base interactions in dinucleotide analog.
Purification and characterization of the purE, purK, and purC gene products: identification of a previously unrecognized energy requirement in the purine biosynthetic pathway.
A new energy requirement in the purine biosynthetic pathway has been established and the gene products of the purE and purK genes thought to be responsible for this activity have been overexpressed and the proteins purified to homogeneity.
Azido auxins: quantitative binding data in maize.
It is found that IAA or NAA, included with this 5-N(3)IAA pretreatment, can protect the sites from blockage, whereas benzoic acid and tryptophan are unable to protect the site, indicating that 5- naphthalene-1-acetic acid specifically labels the auxin sites.
Specificity of the 1-methyladenine receptors in starfish oocytes: synthesis and properties of some 1,8-disubstituted adenines, 1,6-dimethyl-1H-purine, and of the 1-(azidobenzyl)adenines
The low level of activity of the 8-azido derivatives of 1-methyl- and 1-benzyl-adenines, probably due to blocking of the N-7, N-9 region and reduction of the pKa, value, precludes their use as photoaffinity labelling reagents for the receptors of 1 -methyladenine.