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L-Asparaginase from Erwinia Chrysanthemi 3937: cloning, expression and characterization.
Cloning, expression and characterisation of Erwinia carotovora L-asparaginase.
New downstream processing strategy for the purification of monoclonal antibodies from transgenic tobacco plants.
Plant GSTome: structure and functional role in xenome network and plant stress response.
Crystallographic and functional characterization of the fluorodifen-inducible glutathione transferase from Glycine max reveals an active site topography suited for diphenylether herbicides and a…
Engineering thermal stability of l‐asparaginase by in vitro directed evolution
Analysis of the electrostatic potential of the wild‐type enzyme showed that Asp133 is located at a neutral region on the enzyme surface and makes a significant and unfavourable electrostatic contribution to overall stability.
Random mutagenesis methods for in vitro directed enzyme evolution.
- N. Labrou
- BiologyCurrent protein & peptide science
- 18 November 2009
The next sections of this review article focus on recent advances in techniques and methods used for in vitro directed evolution of enzymes using random mutagenesis.
Functional characterization of glutathione S-transferases associated with insecticide resistance in Tetranychus urticae.
Structure‐guided alteration of coenzyme specificity of formate dehydrogenase by saturation mutagenesis to enable efficient utilization of NADP+
- A. Andreadeli, D. Platis, V. Tishkov, V. Popov, N. Labrou
- Biology, ChemistryThe FEBS journal
- 1 August 2008
The results demonstrate that the combined polar interactions and steric factors comprise the main structural determinants responsible for coenzyme specificity.
Characterization of the NAD+ binding site of Candida boidinii formate dehydrogenase by affinity labelling and site-directed mutagenesis.
Results indicate that oADP inactivates FDH by specific reaction at the nucleotide binding site, with negative cooperativity between subunits accounting for the appearance of two phases of inactivation.