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Systematic analysis of protein turnover in primary cells
A better understanding of proteostasis in health and disease requires robust methods to determine protein half-lives. Here we improve the precision and accuracy of peptide ion intensity-basedExpand
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A comprehensive comparison of tools for differential ChIP-seq analysis
TLDR
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/ licenses/by-nc/4.0/). Expand
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Pervasive Protein Thermal Stability Variation during the Cell Cycle
Summary Quantitative mass spectrometry has established proteome-wide regulation of protein abundance and post-translational modifications in various biological processes. Here, we used quantitativeExpand
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Proteome-wide solubility and thermal stability profiling reveals distinct regulatory roles for ATP
Adenosine triphosphate (ATP) plays fundamental roles in cellular biochemistry and was recently discovered to function as a biological hydrotrope. Here, we use mass spectrometry to interrogateExpand
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Creating functional engineered variants of the single-module non-ribosomal peptide synthetase IndC by T domain exchange.
Non-ribosomal peptide synthetases (NRPSs) are enzymes that catalyze ribosome-independent production of small peptides, most of which are bioactive. NRPSs act as peptide assembly lines whereExpand
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Meltome atlas—thermal proteome stability across the tree of life
We have used a mass spectrometry-based proteomic approach to compile an atlas of the thermal stability of 48,000 proteins across 13 species ranging from archaea to humans and covering meltingExpand
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Thermal proteome profiling in bacteria: probing protein state in vivo
Increasing antibiotic resistance urges for new technologies for studying microbes and antimicrobial mechanism of action. We adapted thermal proteome profiling (TPP) to probe the thermostability ofExpand
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Deciphering programs of transcriptional regulation by combined deconvolution of multiple omics layers
Metazoans are crucially dependent on multiple layers of gene regulatory mechanisms which allow them to control gene expression across developmental stages, tissues and cell types. Multiple recentExpand
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Identifying drug targets in tissues and whole blood with thermal-shift profiling
Monitoring drug–target interactions with methods such as the cellular thermal-shift assay (CETSA) is well established for simple cell systems but remains challenging in vivo. Here we introduce tissueExpand
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MapMyFlu: visualizing spatio-temporal relationships between related influenza sequences
TLDR
We present a new web tool, MapMyFlu, which allows to spatially and temporally display influenza viruses related to a query sequence on a Google Map based on BLAST results against the NCBI Influenza Database. Expand
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