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A high-speed atomic force microscope for studying biological macromolecules
The atomic force microscope (AFM) is a powerful tool for imaging individual biological molecules attached to a substrate and placed in aqueous solution. At present, however, it is limited by the
Video imaging of walking myosin V by high-speed atomic force microscopy
This work directly visualize myosin V molecules walking along actin tracks, using high-speed atomic force microscopy, providing corroborative ‘visual evidence’ for previously speculated or demonstrated molecular behaviours, and reveals more detailed behaviours of the molecules, leading to a comprehensive understanding of the motor mechanism.
A high-speed atomic force microscope for studying biological macromolecules.
An AFM that can capture a 100 x 100 pixel(2) image within 80 ms and therefore can generate a movie consisting of many successive images of a sample in aqueous solution, demonstrated by imaging myosin V molecules moving on mica.
High-speed AFM and nano-visualization of biomolecular processes
This article compares high-speed AFM with its competitor (single-molecule fluorescence microscopy) on various aspects and then describes high- Speed AFM instrumentation and imaging studies on biomolecular processes.
High-speed AFM and applications to biomolecular systems.
Theoretical considerations for the highest possible imaging rate of this new microscope are described, and recent imaging studies are highlighted to highlight the current limitation and future challenges to explore.
Guide to video recording of structure dynamics and dynamic processes of proteins by high-speed atomic force microscopy
The protocols are adaptable in general for imaging many proteins and protein–nucleic acid complexes, and examples are described for looking at walking myosin, ATP-hydrolyzing rotorless F1-ATPase and cellulose-hydrosin, and the entire protocol takes 10–15 h, depending mainly on the substrate surface to be used.
Dynamic proportional-integral-differential controller for high-speed atomic force microscopy
In tapping mode atomic force microscopy, the cantilever tip intermittently taps the sample as the tip scans over the surface. This mode is suitable for imaging fragile samples such as biological
Active damping of the scanner for high-speed atomic force microscopy
The scanner that moves the sample stage in three dimensions is a crucial device that limits the imaging rate of atomic force microscopy. This limitation derives mainly from the resonant vibrations of
Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy
High-speed atomic force micropcopy (HS-AFM) movies indicate that, whereas apo-Cas9 adopts unexpected flexible conformations, Cas9–RNA forms a stable bilobed structure and interrogates target sites on the DNA by three-dimensional diffusion.
High‐speed atomic force microscopy for observing dynamic biomolecular processes
The current state of the capability and limitations of high‐speed AFM are described, and possibilities that may break the limitations and lead to the development of a truly useful high‐ Speed AFM for biological sciences are discussed.