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Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria.
Improved broad-host-range plasmid vectors were constructed based on existing plasmids RSF1010 and RK404 and have several additional cloning sites and improved antibiotic-resistance genes which facilitate subcloning and mobilization into various Gram-negative bacteria. Expand
Molecular characterization of cloned avirulence genes from race 0 and race 1 of Pseudomonas syringae pv. glycinea
The identification and cloning of avrB1 provides genetic evidence for a gene-for-gene interaction in the bacterial blight disease of soybean, as avr B1 from race 1 interacts with the soybean disease resistance locus, Rpg1. Expand
Microbial phyllosphere populations are more complex than previously realized
Like other studied ecosystems, microbial phyllosphere communities are more complex than previously thought, based on conventional culture-based methods. Expand
The Role of Pectic Enzymes in Plant Pathogenesis
It is demonstrated that fragments released from the cell wall by pectic enzymes can elicit plant defense reactions and that the highly pectolytic bacteria, Erwinia chrysanthemi and E. carotovora, are amenable to a powerful array of molecular genetic manipulations. Expand
Gene-for-gene complementarity in plant-pathogen interactions.
  • N. Keen
  • Biology, Medicine
  • Annual review of genetics
  • 1990
The cloning of avirulence genes has greatly aided the understanding of plant-pathogen specificity and established firm genetic and biochemical evidence supporting the elicitor-receptor model for recognition of incompatible pathogen races by plants. Expand
New domain motif: the structure of pectate lyase C, a secreted plant virulence factor.
The three-dimensional structure of pectate lyase C from Erwinia chrysanthemi has been solved and refined to a resolution of 2.2 angstroms and suggests that the parallel beta helix motif may occur in a broad spectrum of proteins. Expand
Genome Sequence of the Plant-Pathogenic Bacterium Dickeya dadantii 3937
The sequence of strain 3937, a strain widely used as a model system for research on the molecular biology and pathogenicity of this group of bacteria, is presented. Expand
Structure of a Plant Cell Wall Fragment Complexed to Pectate Lyase C
The results suggest that an arginine, which is invariant in the pectate lyase superfamily, is the amino acid that initiates proton abstraction during the β elimination cleavage of polygalacturonic acid. Expand
Cloned avirulence gene of Pseudomonas syringae pv. glycinea determines race-specific incompatibility on Glycine max (L.) Merr.
A genomic library of Pseudomonas syringae pv. glycinea race 6 DNA was constructed in the mobilizable cosmid vector pLAFR1 and maintained in Escherichia coli HB101. Completeness of the library wasExpand
First crystallographic structure of a xylanase from glycoside hydrolase family 5: implications for catalysis.
The room-temperature structure of xylanase (EC from the bacterial plant pathogen Erwinia chrysanthemi expressed in Escherichia coli, a 45 kDa, 413-amino acid protein belonging to glycosideExpand